PXD001950

PXD001950 is an original dataset announced via ProteomeXchange.

Title	The heparin-binding proteome in nomal pancreas and murine experimental acute pancreatitis, C4PR_LIV
Description	Acute pancreatitis (AP) is an acute inflammation of the pancreas, mainly caused by gallstones and alcohol, and driven by changes in communication between cells. Heparin-binding proteins (HBPs) of the plasma membrane and extracellular matrix play a central role in cell communication. Therefore, we used heparin affinity proteomics to identify the extracellular HBPs in mouse normal pancreas (NP) and in a caerulein mouse model of AP. Many new HBPs (460) were discovered more than doubling their total number to 883. A number of the new HBPs are proteins with well-characterised intracellular functions, e.g. NDUFS4, NDUFS6, but which also have a documented extracellular presence with potential ‘moonlighting’ roles. The HBPs form highly interconnected protein-protein interaction networks in both NP and AP, as well as globally. Thus, HBPs may represent the most interconnected set of extracellular proteins and so those with the greatest regulatory potential. HBPs in NP are associated with biological functions such as molecular transport, cellular movement and tissue architecture that underlie pancreatic homeostasis. However, in AP HBPs are additionally associated with processes such as acute phase response signalling, complement system and mitochondrial dysfunction. By virtue of their extracellular location and heparin binding property, HBPs are easily accessible and are potential biomarkers and drug targets in AP.
HostingRepository	PRIDE
AnnounceDate	2018-12-11
AnnouncementXML	Submission_2019-06-20_03:37:25.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001950
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Philip Brownridge
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-18 04:57:07	ID requested
1	2018-12-11 06:23:11	announced
⏵ 2	2019-06-20 03:48:14	announced	Updated publication reference for PubMed record(s): 31211768.

Nunes QM, Su D, Brownridge PJ, Simpson DM, Sun C, Li Y, Bui TP, Zhang X, Huang W, Rigden DJ, Beynon RJ, Sutton R, Fernig DG, The heparin-binding proteome in normal pancreas and murine experimental acute pancreatitis. PLoS One, 14(6):e0217633(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: mouse, pancreas, LCMSMS

David G Fernig
contact affiliation	Institute of Integrative Biology Biosciences Building Crown Street University of Liverpool Liverpool L69 7ZB UK
contact email	dgfernig@liverpool.ac.uk
lab head
Philip Brownridge
contact affiliation	University of Liverpool
contact email	philipjb@liv.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001950
     1. Label: PRIDE project
     2. Name: The heparin-binding proteome in nomal pancreas and murine experimental acute pancreatitis, C4PR_LIV