PXD001944

PXD001944 is an original dataset announced via ProteomeXchange.

Title	Proteomic interrogation of the cell envelope of Neisseria gonorrhoeae exposed to physiologically relevant environmental cues.
Description	Gonorrhea occurs at high incidence worldwide and has a major impact on reproductive and neonatal health worldwide. Alarmingly, with each new antibiotic introduced for gonorrhea, resistance has emerged, including resistance to penicillin, tetracycline, fluoroquinolones, and recently the third-generation cephalosporins. Treatment options are currently seriously limited and the development of a gonorrhea vaccine is a critical, longterm solution to this problem. Progress on gonorrhea vaccines has been slow, however, in part due to the high number of surface molecules in Neisseria gonorrhoeae (GC) that undergo phase or antigenic variation and a lack of understanding of protective responses. Gonorrhea vaccine development can therefore benefit from a comprehensive, unbiased approach for antigen discovery. Here we identified cell envelop proteins from Neisseria gonorrhoeae exposed to physiology relevant conditions: presence of human serum, iron limitation and anaerobic growth.
HostingRepository	PRIDE
AnnounceDate	2016-05-09
AnnouncementXML	Submission_2016-05-09_02:19:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandra Sikora
SpeciesList	scientific name: Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090); NCBI TaxID: 242231;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; L-cysteine methyl disulfide; monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-17 02:29:54	ID requested
⏵ 1	2016-05-09 02:19:51	announced

Zielke RA, Wierzbicki IH, Baarda BI, Gafken PR, Soge OO, Holmes KK, Jerse AE, Unemo M, Sikora AE, Proteomics-driven Antigen Discovery for Development of Vaccines Against Gonorrhea. Mol Cell Proteomics, 15(7):2338-55(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Neisseria gonorrhoeae, cell envelope, quantitative proteomics, iTRAQ

Aleksandra Sikora
contact affiliation	Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, Oregon 97331, USA
contact email	aleksandra.sikora@oregonstate.edu
lab head
Aleksandra Sikora
contact affiliation	College of Pharmacy
contact email	aleksandra.sikora@oregonstate.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001944

PRIDE project URI

• PRIDE
  1. PXD001944
     1. Label: PRIDE project
     2. Name: Proteomic interrogation of the cell envelope of Neisseria gonorrhoeae exposed to physiologically relevant environmental cues.