PXD001934

PXD001934 is an original dataset announced via ProteomeXchange.

Title	Cdc42 controls DC-maturation
Description	Cell division cycle 42 (Cdc42) is a member of the Rho GTPase family and has pivotal functions in actin organization, cell migration and proliferation. Cdc42 has been shown to regulate antigen (Ag)-uptake in immature dendritic cells (DC) and controls their migration from tissues to lymph nodes. Previous reports demonstrated that Cdc42 is inactivated upon DC-maturation to avoid continued Ag-acquisition. To further study the molecular mechanisms of DC-control by Cdc42, we used bone marrow-derived DCs from Cdc42-deficient mice. We show that Cdc42-deficient DCs are phenotypically mature without additional maturation stimuli, as they upregulate CD86 from intracellular storages to the cell surface. They also accumulate invariant chain (Ii)-MHC class II complexes at the cell surface, which cannot efficiently present peptide Ag for priming of Ag-specific CD4 T cells. Lack of Cdc42 in immature DCs does not allow MHC class II maturation, as lysosomal Cathepsins are lost into the supernatant and Ii-MHC class II complexes cannot mature. Therefore Cdc42-deficient DCs are "pseudomature" and lose most functional hallmarks of antigen-presenting cells. Our results propose that Cdc42 keeps DCs in an immature state, while downregulation of Cdc42-activity during maturation facilitates generation of CD86+MHCII+ mature DCs.
HostingRepository	PRIDE
AnnounceDate	2015-10-12
AnnouncementXML	Submission_2016-03-21_03:10:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-16 01:28:50	ID requested
1	2015-10-12 03:00:21	announced
⏵ 2	2016-03-21 03:10:14	announced	Updated publication reference for PubMed record(s): 26553928.

Schulz AM, Stutte S, Hogl S, Luckashenak N, Dudziak D, Leroy C, Forn, é I, Imhof A, M, ü, ller SA, Brakebusch CH, Lichtenthaler SF, Brocker T, Cdc42-dependent actin dynamics controls maturation and secretory activity of dendritic cells. J Cell Biol, 211(3):553-67(2015) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: LC-MS, label-free, Cdc42, dendritic cells

Thomas Brocker
contact affiliation	Institute for Immunology Ludwig-Maximilians-University D-80336 Munich Germany
contact email	tbrocker@med.uni-muenchen.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/10/PXD001934

PRIDE project URI

• PRIDE
  1. PXD001934
     1. Label: PRIDE project
     2. Name: Cdc42 controls DC-maturation