PXD001932

PXD001932 is an original dataset announced via ProteomeXchange.

Title	p65-HaloTag copurifying proteins in human 293T cells
Description	In effort to develop methodology for targeted top down mass spectrometry of NF kappa B p65 from human cells, we evaluated the utility of HaloTag for purification and analysis of recombinant protein. During our study, two datasets of bottom up LC-MS/MS were generated: one from in-gel digestion of the predominant band following p65-HaloTag purification, another from in-solution digestion of all the proteins present in a p65-HaloTag purification. p65-HaloTag copurifying proteins identified in our datasets include the known interactors c-Rel, NF-kappaB p105, NF-kappaB p100, and NF-kappaB inhibitor beta. Over 100 proteins were identified by at least two peptides using a Mascot ion cut-off score of 30.
HostingRepository	PRIDE
AnnounceDate	2018-10-19
AnnouncementXML	Submission_2018-10-19_01:21:47.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001932
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	John Savaryn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ FT; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-13 08:35:26	ID requested
⏵ 1	2018-10-19 01:21:48	announced

Savaryn JP, Skinner OS, Fornelli L, Fellers RT, Compton PD, Terhune SS, Abecassis MM, Kelleher NL, Targeted analysis of recombinant NF kappa B (RelA/p65) by denaturing and native top down mass spectrometry. J Proteomics, 134():76-84(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: p65, RelA, NF kappa B, human, 293T

Neil Kelleher
contact affiliation	Northwestern University, Evanston, Illinois, USA
contact email	n-kelleher@northwestern.edu
lab head
John Savaryn
contact affiliation	Northwestern University
contact email	john.savaryn@northwestern.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD001932

PRIDE project URI

• PRIDE
  1. PXD001932
     1. Label: PRIDE project
     2. Name: p65-HaloTag copurifying proteins in human 293T cells