PXD001930

PXD001930 is an original dataset announced via ProteomeXchange.

Title	Copper stress-induced changes in leaf soluble proteome of Cu-sensitive and tolerant Agrostis capillaris L. populations
Description	Changes in leaf soluble proteome were explored in 3-month-old plants of Cu-tolerant (metallicolous, M) and sensitive (non-metallicolous, NM) Agrostis capillaris L. populations exposed to increasing Cu concentrations (1-50 µM). Aims of the study were to investigate (1) differential protein expression in NM and M leaves under increasing Cu exposure, and (2) molecular mechanisms underlying higher Cu tolerance in M plants. Plants were cultivated on perlite (CuSO4 spiked-nutrient solution); fresh leaf aliquots were frozen in liquid nitrogen. Soluble leaf proteins were extracted by the trichloroacetic acid/acetone procedure, and separated with 2-DE (linear 4-7 pH gradient). Analysis of CCB-stained gels (PDQuest) reproducibly detected 214 spots, of which 107 were analyzed using LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2016-04-12
AnnouncementXML	Submission_2016-04-12_03:21:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Claverol Claverol
SpeciesList	scientific name: Agrostis capillaris (Colonial bentgrass) (Agrostis tenuis); NCBI TaxID: 204232;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LCQ Deca XP Plus

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-13 05:15:19	ID requested
⏵ 1	2016-04-12 03:21:22	announced

Hego E, Vilain S, Barr, é A, Claverol S, Dupuy JW, Lalanne C, Bonneu M, Plomion C, Mench M, Copper stress-induced changes in leaf soluble proteome of Cu-sensitive and tolerant Agrostis capillaris L. populations. Proteomics, 16(9):1386-97(2016) [pubmed]

curator keyword: Biological

submitter keyword: Plant proteomics

LC-MS/MS

Agrostis capillaris

Cu-tolerance

Photosynthesis

Heat-shock protein

Marc Bonneu
contact affiliation	Plateforme Proteome Centre Genomique Fonctionnelle Bordeaux 146 rue Leo Saignat 33076 Bordeaux Cedex France
contact email	marc.bonneu@ipb.fr
lab head
Claverol Claverol
contact affiliation	Plateforme Proteome
contact email	stephane.claverol@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD001930

PRIDE project URI

• PRIDE
  1. PXD001930
     1. Label: PRIDE project
     2. Name: Copper stress-induced changes in leaf soluble proteome of Cu-sensitive and tolerant Agrostis capillaris L. populations