PXD001909

PXD001909 is an original dataset announced via ProteomeXchange.

Title	Proteomic Identification of Nuclear Processes Manipulated by Cytomegalovirus Early during Infection
Description	Human cytomegalovirus (HCMV) is a herpesvirus that is ubiquitously distributed world-wide and causes life-threating disease upon immunosuppression. HCMV expresses numerous proteins that function to establish an intracellular environment that supports viral replication. Like most DNA viruses, HCMV manipulates processes within the nucleus. We have quantified changes in the host cell nuclear proteome at 24 hpi following infection with a clinical viral isolate. We have combined SILAC with multiple stages of fractionation to define changes. Tryptic peptides were analyzed by RP-HPLC combined with LC-MS/MS on an LTQ Orbitrap Velos massspectrometer. Data from three biological replicates were processed with MaxQuant. A total of 1281 cellular proteins were quantified and 77 were found to be significantly differentially expressed. In addition, we observed 36 viral proteins associated with the nucleus. Diverse biological processes were significantly altered including increased aspects of cell cycling, mRNA metabolism, and nucleocytoplasmic transport while decreased immune responses. We validated changes for several proteins including a subset of classical nuclear transport proteins. In addition, we demonstrated that disruption of these import factors is inhibitory to HCMV replication. Overall, we have identified HCMV-induced changes in the nuclear proteome and uncovered several processes that are important for infection.
HostingRepository	PRIDE
AnnounceDate	2015-06-23
AnnouncementXML	Submission_2015-06-23_04:52:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominique Carter
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human cytomegalovirus (HHV-5) (Human herpesvirus 5); NCBI TaxID: 10359;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; 6x(13)C labeled residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-12 02:55:19	ID requested
⏵ 1	2015-06-23 04:52:03	announced

Carter DM, Westdorp K, Noon KR, Terhune SS, Proteomic identification of nuclear processes manipulated by cytomegalovirus early during infection. Proteomics, 15(12):1995-2005(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: HCMV, cytomegalovirus, quantitative proteomics, SILAC

Scott Terhune
contact affiliation	Biotechnology and Bioengineering Center, Deptartment of Microbiology, Medical College of Wisconsin
contact email	sterhune@mcw.edu
lab head
Dominique Carter
contact affiliation	Medical College of Wisconsin
contact email	docarter@mcw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001909
     1. Label: PRIDE project
     2. Name: Proteomic Identification of Nuclear Processes Manipulated by Cytomegalovirus Early during Infection