PXD001897

PXD001897 is an original dataset announced via ProteomeXchange.

Title	Proteomics method for secretome analysis of filamentous fungi
Description	We describe a simple, plate-based method to analyze the secretomes of microorganisms growing on insoluble substrates that allows harsh sample preparation methods promoting desorption, and subsequent identification, of substrate-bound proteins, while minimizing contamination with non-secreted proteins from leaking or lysed cells. The method described here enables rapid large-scale comparative studies of the secretomes of filamentous fungi and other microorganisms growing on a variety of solid substrates, which eventually may have implications for our understanding of enzymatic lignocellulose degradation.
HostingRepository	PRIDE
AnnounceDate	2015-10-26
AnnouncementXML	Submission_2015-10-26_05:14:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magnus Arntzen
SpeciesList	scientific name: Hypocrea jecorina (strain QM6a) (Trichoderma reesei); NCBI TaxID: 431241;
ModificationList	deamidated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-10 02:28:38	ID requested
⏵ 1	2015-10-26 05:14:36	announced

Bengtsson O, Arntzen M Ø, Mathiesen G, Skaugen M, Eijsink VGH, A novel proteomics sample preparation method for secretome analysis of Hypocrea jecorina growing on insoluble substrates. J Proteomics, 131():104-112(2016) [pubmed]

curator keyword: Biological

submitter keyword: secretome, sample preparation, fungi, substrate, comparative analysis, Hypocrea jecorina, Trichoderma reesei

Vincent Eijsink
contact affiliation	Norwegian University of Life Sciences, NMBU, Department of Chemistry, Biotechnology and Food Science, P.O. Box 5003, NO-1432 Aas, Norway
contact email	vincent.eijsink@nmbu.no
lab head
Magnus Arntzen
contact affiliation	Norwegian University of Life Sciences
contact email	magnus.arntzen@nmbu.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/10/PXD001897

PRIDE project URI

• PRIDE
  1. PXD001897
     1. Label: PRIDE project
     2. Name: Proteomics method for secretome analysis of filamentous fungi