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PXD001881

PXD001881 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleArabidopsis endomembrane enrichments: Affinity enrichment of YFP-RABG3f
DescriptionOur aim was to dissect subcellular trafficking routes by enriching for partially overlapping subpopulations of endosomal proteomes associated with endomembrane markers. We selected RABD2a/ARA5, RABF2b/ARA7, RABF1/ARA6, and RABG3f as markers for combinations of the Golgi, trans-Golgi Network (TGN), Early Endosomes (EE), secretory vesicles, Late Endosomes (LE), Multivesicular Bodies (MVB) compartments and tonoplast. As comparisons we used Golgi transport 1 (GOT1), which localises to the Golgi, Clathrin Light Chain 2 (CLC2) labelling Clathrin-coated vesicles and pits and the Vesicle Associated Membrane Protein 711 (VAMP711) present at the tonoplast. We developed an easy-to-use method by refining published protocols based on affinity purification of fluorescent fusion constructs to these seven subcellular marker proteins in Arabidopsis thaliana seedlings.
HostingRepositoryPRIDE
AnnounceDate2015-04-22
AnnouncementXMLSubmission_2015-04-27_03:15:46.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD001881
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlex Jones
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListOxidation; Carbamidomethyl
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-03-06 05:32:34ID requested
12015-04-22 07:13:49announced
22015-04-27 03:15:47announcedUpdated publication reference for PubMed record(s): 25900983.
Publication List
Heard W, Sklen, á, ř J, Tom, é DF, Robatzek S, Jones AM, Identification of Regulatory and Cargo Proteins of Endosomal and Secretory Pathways in Arabidopsis thaliana by Proteomic Dissection. Mol Cell Proteomics, 14(7):1796-813(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Arabidopsis, affnity purification, clathin, small GTPases,
Contact List
Alexandra M. E. Jones
contact affiliationSchool of Life Sciences, University of Warwick
contact emailalex.jones@warwick.ac.uk
lab head
Alex Jones
contact affiliationUniversity of Warwick
contact emailalex.jones@warwick.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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