PXD001875

PXD001875 is an original dataset announced via ProteomeXchange.

Title	The prion protein contributes to epithelial-to-mesenchymal transition in the mouse NMuMG epithelial cell model
Description	The physiological function of the prion protein (PrP) has remained elusive despite its widely recognized role in neurodegenerative diseases and sustained efforts to understand its molecular biology. On the basis of its evolutionary relationship to ZIP zinc transporters, protein-protein interactions it engages in, and the characteristics of a gastrulation arrest phenotype in a PrP-deficient model, we considered that PrP may contribute to the morphogenetic reprogramming of cells underlying epithelial-to-mesenchymal (EMT) transitions. We have conducted three global proteomics analyses to (1) identify proteins whose levels are changed during EMT in wild-type NMuMG cells; and determine proteins whose levels are affected by (2) stable knockdown or (3) transient knockdown of the prion protein relative to levels observed in wild-type NMuMG cells. We observed a highly significant correlation between proteins, which are changed during EMT or following PrP knockdown.
HostingRepository	PRIDE
AnnounceDate	2015-07-10
AnnouncementXML	Submission_2015-08-25_03:44:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerold Schmitt-Ulms
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-pyridylethyl-L-cysteine; TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-06 01:17:35	ID requested
1	2015-07-10 09:07:31	announced
⏵ 2	2015-08-25 03:44:23	announced	Updated publication reference for PubMed record(s): 26288071.

Mehrabian M, Brethour D, Wang H, Xi Z, Rogaeva E, Schmitt-Ulms G, The Prion Protein Controls Polysialylation of Neural Cell Adhesion Molecule 1 during Cellular Morphogenesis. PLoS One, 10(8):e0133741(2015) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: prion protein, epithelial-to-mesenchymal transition, Orbitrap fusion, TMT

Gerold Schmitt-Ulms
contact affiliation	Tanz Centre for Research in Neurodegnerative Diseases (Tanz CRND) Department of Laboratory Medicine and Pathobiology University of Toronto
contact email	g.schmittulms@utoronto.ca
lab head
Gerold Schmitt-Ulms
contact affiliation	University of Toronto
contact email	g.schmittulms@utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/07/PXD001875

PRIDE project URI

• PRIDE
  1. PXD001875
     1. Label: PRIDE project
     2. Name: The prion protein contributes to epithelial-to-mesenchymal transition in the mouse NMuMG epithelial cell model