PXD001873

PXD001873 is an original dataset announced via ProteomeXchange.

Title	MCF-7 phosphoproteome LC-MS/MS
Description	MCF-7 cells were stimulated with TNF-alpha in order to identify IKKb substrates. conditions: TNF alpha stimulation TNF alpha stimulation + SC-514 IKK (kinase dead mutant) + TNF alpha stimulation IKK(WT) + TNF alpha stimulation basal Already validated IKK substrates were used to train random forest and to predict new substrates. Among other interesting candidates we validated AEG-1 (S298) as an IKKb substrate. We provide evidence that IKKb-mediated AEG-1 phosphorylation is essential for IkBa degradation as well as NF-kB-dependent gene expression and cell proliferation, which correlate with cancer patient survival in vivo. (replicate 1 out of at least 2)
HostingRepository	PRIDE
AnnounceDate	2018-10-17
AnnouncementXML	Submission_2018-10-17_06:07:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hendrik Nolte
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-06 01:13:30	ID requested
⏵ 1	2018-10-17 06:07:01	announced

Krishnan RK, Nolte H, Sun T, Kaur H, Sreenivasan K, Looso M, Offermanns S, Kr, ü, ger M, Swiercz JM, substrate. Nat Commun, 6():6658(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: MCF-7 , phosphoproteome, TNF stimulation, IKKb

Marcus Krüger
contact affiliation	Max Planck Institute Bad Nauheim for Heart and Lung Research Ludwigstrasse 42 Bad Nauheim, Germany
contact email	marcus.krueger@mpi-bn.mpg.de
lab head
Hendrik Nolte
contact affiliation	HN
contact email	hendrik.nolte@mpi-bn.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD001873

PRIDE project URI

• PRIDE
  1. PXD001873
     1. Label: PRIDE project
     2. Name: MCF-7 phosphoproteome LC-MS/MS