PXD001870

PXD001870 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Analysis of Plasma Membrane, Endosomal and Cytoplasmic fractions of mouse embryonic fibroblast
Description	This project aimed to identify novel endosomal proteins and therefore cellular fractionation experiments were performed. Adherent mouse embryonic fibroblast were scraped on ice into hypotonic lysis buffer (10 mM HEPES-KOH pH 7.2, 0.25 M sucrose, 1 mM EDTA, 1 mM MgOAc and protease & phosphatase inhibitors (PhosSTOP and Complete mini tablets from Roche). Cell membranes were fragmented with French Press and nuclei removed with 10 min 1000 x g centrifugation. Plasma membrane fraction was collected with 10 min 10 000 x g centrifugation and endosomal/cytoplasmic fractions with 1h 100,000 x g centrifugation. Membrane fractions were washed at least once with lysis buffer and cytoplasmic fractions were centrifuged twice. All fractionation steps were performed at +4°C or on ice. All fractions were dissolved in sample buffer for immunoblotting
HostingRepository	PRIDE
AnnounceDate	2015-10-14
AnnouncementXML	Submission_2015-10-14_06:49:05.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001870
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Guillaume Jacquemet
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-05 01:13:15	ID requested
⏵ 1	2015-10-14 06:49:18	announced

Publication List

Alanko J, Mai A, Jacquemet G, Schauer K, Kaukonen R, Saari M, Goud B, Ivaska J, Integrin endosomal signalling suppresses anoikis. Nat Cell Biol, 17(11):1412-21(2015) [pubmed]

Alanko J, Mai A, Jacquemet G, Schauer K, Kaukonen R, Saari M, Goud B, Ivaska J, Integrin endosomal signalling suppresses anoikis. Nat Cell Biol, 17(11):1412-21(2015) [pubmed]

Keyword List

curator keyword: Biomedical, Biological
submitter keyword: Cell fractionation, endosomes, plasma membrane, cytosol, mouse embryonic fibroblasts

curator keyword: Biomedical, Biological

submitter keyword: Cell fractionation, endosomes, plasma membrane, cytosol, mouse embryonic fibroblasts

Contact List

Johanna Ivaska
contact affiliation	University of Turku, Finland
contact email	joivaska@utu.fi
lab head
Guillaume Jacquemet
contact affiliation	University of Turku
contact email	guillaume.jacquemet@btk.fi
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/10/PXD001870
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/10/PXD001870

PRIDE project URI

Repository Record List

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