PXD001863

PXD001863 is an original dataset announced via ProteomeXchange.

Title	Identification of myristoylated proteins in Human cell lines (HEK293, HeLa, MCF7)
Description	Multi-functional capture reagents reported here enable robust identification of metabolically-tagged myristoylated proteomes with unprecedented confidence resulting from the combination of chemical probe-based enrichment, and release and direct detection of lipid-modified peptides by MS. Whilst capture reagents containing enzymatically cleavable linkers have been previously reported they typically require an extra proteolytic step and their capacity to enable detection of lipidated peptides has not been demonstrated. Herein, we report the largest database (85 counts) of experimentally validated human proteins that are myristoylated at an endogenous level in living cells. Furthermore, we demonstrate the first profile of myristoylation in a living multicellular organism and the confident identification of over 50 novel targets. Importantly, this is also the first example of analysis of any protein lipidation event during development. Our methodology is novel in analytical/chemical approach, and provides quantitative and dynamic information. This submission concerns myristoylated proteomes of human origin.
HostingRepository	PRIDE
AnnounceDate	2015-04-01
AnnouncementXML	Submission_2015-04-01_00:31:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	myristoylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-03 07:44:30	ID requested
1	2015-03-30 04:08:38	announced
⏵ 2	2015-04-01 00:31:27	announced	Updated project metadata.

Broncel M, Serwa RA, Ciepla P, Krause E, Dallman MJ, Magee AI, Tate EW, Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic profiling of protein lipidation during vertebrate development. Angew Chem Int Ed Engl, 54(20):5948-51(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, Protein myristoylation, Myristoylated peptides, chemical probes, LC-MS/MS

Edward W. Tate
contact affiliation	Department of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ, UK
contact email	e.tate@imperial.ac.uk
lab head
Remigiusz Serwa
contact affiliation	Imperial College London
contact email	r.serwa@imperial.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/03/PXD001863

PRIDE project URI

• PRIDE
  1. PXD001863
     1. Label: PRIDE project
     2. Name: Identification of myristoylated proteins in Human cell lines (HEK293, HeLa, MCF7)