PXD001857

PXD001857 is an original dataset announced via ProteomeXchange.

Title	Rest recruits neuronal Tet3 to induce chromatin remodeling and gene expression
Description	Ten-eleven translocation (Tet) hydroxylases (Tet1-3) oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). In neurons increased 5hmC levels within gene bodies correlate positively with gene expression. The mechanisms controlling Tet activity and 5hmC levels are poorly understood. In particular, it is not known how the neuronal Tet3 isoform lacking a DNA binding domain is targeted to the DNA. To identify factors binding to Tet3 we screened for proteins that co-precipitate with Tet3 from mouse retina and identified the transcriptional repressor Rest as a highly enriched Tet3-specific interactor. Rest was able to enhance Tet3 hydroxylase activity after co-expression and overexpression of Tet3 activated transcription of Rest-target genes. Moreover, we found that Tet3 also interacts with Nsd3 and two other H3K36 methyltransferases and is able to induce H3K36 trimethylation. We propose a mechanism for transcriptional activation in neurons that involves Rest-guided targeting of Tet3 to the DNA for directed 5hmC-generation and Nsd3-mediated H3K36 trimethylation.
HostingRepository	PRIDE
AnnounceDate	2015-04-02
AnnouncementXML	Submission_2015-04-02_01:33:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Eisen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue; monosodium salt
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-02 07:53:00	ID requested
⏵ 1	2015-04-02 01:33:16	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: REST TET3

Stylianos Michalakis
contact affiliation	LMU Muenchen
contact email	stylianos.michalakis@cup.uni-muenchen.de
lab head
David Eisen
contact affiliation	LMU München
contact email	David.Eisen@cup.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/04/PXD001857

PRIDE project URI

• PRIDE
  1. PXD001857
     1. Label: PRIDE project
     2. Name: Rest recruits neuronal Tet3 to induce chromatin remodeling and gene expression