PXD001853

PXD001853 is an original dataset announced via ProteomeXchange.

Title	Light-sensing via H2O2 and a peroxiredoxin
Description	Light controls control a vast array of biological processes, including cell and organelle motility, stress responses, organismal development and the entrainment of circadian rhythms, that maintain diurnal cycles of activity in organisms from cyanobacteria to humans. Recent studies indicate that a type of antioxidant and signaling proteins, peroxiredoxins, sustain circadian rhythms independent of characterized circadian pacemakers in organisms from all the three kingdoms of life, suggesting a role for H2O2 production in circadian clocks. Whereas many circadian clocks involve photosensitive pigments such as melanopsin and cryptochromes it is unclear whether peroxiredoxins can respond to light stimuli and how they interact with global signaling networks regulating e.g. clocks and aging, such as cyclic AMP (cAMP)/protein kinase A (PKA). In yeast, that lacks decidated photoreceptors, blue light induces cAMP-PKA-dependent, nuclear accumulation of a transcription factor, Msn2. However, the mechanism by which light represses pathway activity to stimulate Msn2 nuclear translocation is unknown. Here we identify increased H2O2–production via a conserved peroxisomal oxidase as the cause of light-induced Msn2 nuclear concentration. The H2O2 signal is transduced by the catalytic cysteines of the peroxiredoxin Tsa1 that relieve Msn2 from inhibitory PKA phosphorylation causing its nuclear accumulation. We propose that yeast senses light via H2O2 and a peroxiredoxin to inhibit cAMP/PKA activity and our data form a framework for the study of light responses in cells lacking dedicated light receptors and cAMP-controlled biological rhythms in multicellular organisms.
HostingRepository	PRIDE
AnnounceDate	2018-07-04
AnnouncementXML	Submission_2018-07-04_00:30:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wolfgang Reiter
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-02-27 07:27:43	ID requested
1	2017-02-01 15:12:39	announced
2	2017-03-29 00:18:26	announced	Updated publication reference for PubMed record(s): 28337980.
⏵ 3	2018-07-04 00:30:31	announced	Updated project metadata.

Bodvard K, Peeters K, Roger F, Romanov N, Igbaria A, Welkenhuysen N, Palais G, Reiter W, Toledano MB, K, ä, ll M, Molin M, Light-sensing via hydrogen peroxide and a peroxiredoxin. Nat Commun, 8():14791(2017) [pubmed]

curator keyword: Biological

submitter keyword: Yeast, Msn2, oxidative stress, H2O2, LC-MSMS

Wolfgang Ludwig Reiter
contact affiliation	Max F.Perutz Laboratories (MFPL), University of Vienna; MFPL Mass Spectrometry Facility
contact email	reiterw6@univie.ac.at
lab head
Wolfgang Reiter
contact affiliation	MAX F. PERUTZ LABORATORIES - University of Vienna
contact email	wolfgang.l.reiter@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD001853

PRIDE project URI

• PRIDE
  1. PXD001853
     1. Label: PRIDE project
     2. Name: Light-sensing via H2O2 and a peroxiredoxin