PXD001846

PXD001846 is an original dataset announced via ProteomeXchange.

Title	SILAC Mass Spectrometry of U2AF2 Interactome in Resting and Activated CD4 T cells
Description	T cell activation leads to dramatic changes in cellular phenotype. We used activated human CD4 T cells to study how RNA binding proteins define the post-transcriptional landscape. Using RIPseq, we identified the RNA interactome of U2AF2 and show that U2AF2 binds the majority of transcripts that are differentially expressed and/or alternatively spliced during activation. Using RIP mass spectrometry, a unique protein interactome centered on U2AF2 is assembled by activation and comprised of both directly bound central members (RNAse-resistant) and indirectly bound peripheral members (RNAse-sensitive). Knocking down specific U2AF2 interactome members (U2AF1, SYNCRIP, SRRM2, ILF2) selectively affects cytokine secretion and activation markers. The expression and/or alternative splicing of transcripts important for immune cell function are also affected by knocking down these interactome members, both peripheral and central. Furthermore, we show that knockdown of interactome members can affect the proteins and transcripts bound to U2AF2, altering the transcriptome of activated T cells. Our work highlights the importance of understanding the assembly of RNA binding protein complexes as regulators of T cell activation and function.
HostingRepository	PRIDE
AnnounceDate	2016-04-27
AnnouncementXML	Submission_2016-04-27_03:53:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Whisenant
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2015-02-26 02:06:49	ID requested
⏵ 1	2016-04-27 03:53:57	announced

Whisenant TC, Peralta ER, Aarreberg LD, Gao NJ, Head SR, Ordoukhanian P, Williamson JR, Salomon DR, The Activation-Induced Assembly of an RNA/Protein Interactome Centered on the Splicing Factor U2AF2 Regulates Gene Expression in Human CD4 T Cells. PLoS One, 10(12):e0144409(2015) [pubmed]

curator keyword: Biological

submitter keyword: CD4 T cell activation/mRNA splicing/RNA-Binding Proteins/U2AF

Daniel R. Salomon, M.D.
contact affiliation	Professor Department of Molecular and Experimental Medicine The Scripps Research Institute 10550 North Torrey Pines Road La Jolla, CA 92037
contact email	dsalomon@scripps.edu
lab head
Thomas Whisenant
contact affiliation	The Scripps Research Institute
contact email	thomasw@scripps.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD001846

PRIDE project URI

• PRIDE
  1. PXD001846
     1. Label: PRIDE project
     2. Name: SILAC Mass Spectrometry of U2AF2 Interactome in Resting and Activated CD4 T cells