PXD001843 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protein Interactome of U2AF2 during T cell activation |
Description | T cell activation leads to dramatic changes in cellular phenotype. We used activated human CD4 T cells to study how RNA binding proteins define the post-transcriptional landscape. Using RIPseq, we identified the RNA interactome of U2AF2 and show that U2AF2 binds the majority of transcripts that are differentially expressed and/or alternatively spliced during activation. Using RIP mass spectrometry, a unique protein interactome centered on U2AF2 is assembled by activation and comprised of both directly bound central members (RNAse-resistant) and indirectly bound peripheral members (RNAse-sensitive). Knocking down specific U2AF2 interactome members (U2AF1, SYNCRIP, SRRM2, ILF2) selectively affects cytokine secretion and activation markers. The expression and/or alternative splicing of transcripts important for immune cell function are also affected by knocking down these interactome members, both peripheral and central. Furthermore, we show that knockdown of interactome members can affect the proteins and transcripts bound to U2AF2, altering the transcriptome of activated T cells. Our work highlights the importance of understanding the assembly of RNA binding protein complexes as regulators of T cell activation and function. |
HostingRepository | PRIDE |
AnnounceDate | 2016-04-27 |
AnnouncementXML | Submission_2016-04-27_03:45:42.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thomas Whisenant |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-02-25 03:45:39 | ID requested | |
⏵ 1 | 2016-04-27 03:45:44 | announced | |
Publication List
Whisenant TC, Peralta ER, Aarreberg LD, Gao NJ, Head SR, Ordoukhanian P, Williamson JR, Salomon DR, The Activation-Induced Assembly of an RNA/Protein Interactome Centered on the Splicing Factor U2AF2 Regulates Gene Expression in Human CD4 T Cells. PLoS One, 10(12):e0144409(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: CD4 T cells, U2AF, RNA-binding proteins, RIP mass spectrometry |
Contact List
Daniel R. Salomon, M.D. |
contact affiliation | Professor Department of Molecular and Experimental Medicine The Scripps Research Institute 10550 North Torrey Pines Road La Jolla, CA 92037 |
contact email | dsalomon@scripps.edu |
lab head | |
Thomas Whisenant |
contact affiliation | The Scripps Research Institute |
contact email | thomasw@scripps.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001843
- Label: PRIDE project
- Name: Protein Interactome of U2AF2 during T cell activation