PXD001841

PXD001841 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation of S.pombe Rap1 analysed by LC-MS/MS
Description	Identification and semi-quantitation of phosphorylation sites on Schizosaccharomyces pombe DNA-binding protein Rap1. A Rap1 construct was cultured in homozygous diploid temperature-sensitive pat1-114 mutant strain of S. pombe cells (time points: 3.5 h and 4.5h), and was purified by anti-PK immunoprecipitation. The resulting samples were run into a 1D SDS-PAGE gel with the target band excised, tryptically digested and analysed by LC-MS/MS using a Velos Orbitrap mass spectrometer.
HostingRepository	PRIDE
AnnounceDate	2016-04-27
AnnouncementXML	Submission_2016-04-27_03:59:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Thompson
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-02-24 08:35:06	ID requested
⏵ 1	2016-04-27 03:59:09	announced

Amelina H, Subramaniam S, Moiseeva V, Armstrong CA, Pearson SR, Tomita K, Telomere protein Rap1 is a charge resistant scaffolding protein in chromosomal bouquet formation. BMC Biol, 13():37(2015) [pubmed]

curator keyword: Biological

submitter keyword: Schizosaccharomyces pombe, Rap1, LC-MSMS, phosphorylation

Andrew James Thompson
contact affiliation	Proteomics and Metabolomics Core Facility, Department of Cancer Biology, The Institute of Cancer Research, London, UK.
contact email	andrew.thompson@icr.ac.uk
lab head
Andrew Thompson
contact affiliation	The Institute of Cancer Research
contact email	MSCorefacility@icr.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001841
     1. Label: PRIDE project
     2. Name: Phosphorylation of S.pombe Rap1 analysed by LC-MS/MS