PXD001837

PXD001837 is an original dataset announced via ProteomeXchange.

Title	Erythrocyte soluble proteome analysis in hemolytic anaemia patients
Description	We investigated the RBCs of a hemolytic anemia patient using quantitative proteomics. We used stable isotope dimethyl labeling to accurately quantify the RBC proteins. As controls, 1) samples of four healthy subjects were taken to account for normal variation in healthy individuals, and 2) samples of two non-spherocytic hemolytic anemia patients were taken to account for differences in protein levels due to elevated reticulocyte content. We used a combination of strong cation exchange (SCX) chromatography with nanoLC-MS/MS, enabling quantification of RBC proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:23:24.034.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Barasa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-02-24 00:20:58	ID requested
1	2016-09-26 23:23:29	announced
⏵ 2	2024-10-22 04:23:42	announced	2024-10-22: Updated project metadata.

10.1002/prca.201500130;

Barasa BA, van Oirschot BA, Bianchi P, van Solinge WW, Heck AJ, van Wijk R, Slijper M, Proteomics reveals reduced expression of transketolase in pyrimidine 5'-nucleotidase deficient patients. Proteomics Clin Appl, 10(8):859-69(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: proteomics, hemolytic anema,red blood cell

Albert J R Heck
contact affiliation	Biomolecular Mass Spectrometry
contact email	a.j.r.heck@uu.nl
lab head
Benjamin Barasa
contact affiliation	Universiteit Utrecht
contact email	b.barasa@yahoo.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD001837

PRIDE project URI

• PRIDE
  1. PXD001837
     1. Label: PRIDE project
     2. Name: Erythrocyte soluble proteome analysis in hemolytic anaemia patients