<<< Full experiment listing

PXD001836

PXD001836 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleVitrification of embryos modifies foetal placenta proteome. LCMSMS
DescriptionVery limited information about the post-implantatory effects of vitrification has been published till now. In a previous study, we observed that vitrification procedure introduced transcriptomic and proteomic modifications in the rabbit foetal placenta at the middle of gestation. In addition, a reduction in maternal placenta and foetal weight was also detected. In order to know if these alterations were temporary or not, the aim of the current study was to analyse the proteomic profile of rabbit foetal placenta at two different times of gestation: Day 14 and Day 24. We performed a comparative 2D-DIGE analysis and found that placentas from vitrified embryos expressed eleven different proteins at Day 14 and thirteen at Day 24. The alteration of serum albumin, isocitrate dehydrogenase 1 [NADP+] and phosphoglycerate mutase 1 were maintained both Day 14 and Day 24. Our results demonstrate that vitrification is not neutral, and induce a substantial alteration of placental protein expression, even at the end of gestation. But, apart from short-term effects observed on embryos, could vitrification entail long-term consequences in those foetuses that are going to be born? Further studies will determine if alterations observed could be the set point of future modifications to manifest into adulthood
HostingRepositoryPRIDE
AnnounceDate2015-08-11
AnnouncementXMLSubmission_2015-08-11_03:09:51.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD001836
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLuz Valero
SpeciesList scientific name: Oryctolagus cuniculus algirus; NCBI TaxID: 230741;
ModificationListOxidation; Carbamidomethyl; Deamidated
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-02-23 06:04:05ID requested
12015-08-11 03:09:52announced
Publication List
Saenz-de-Juano MD, Vicente JS, Hollung K, Marco-Jim, é, nez F, Effect of Embryo Vitrification on Rabbit Foetal Placenta Proteome during Pregnancy. PLoS One, 10(4):e0125157(2015) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Cryopreservation
Embryos
rabbit
Placenta
Proteome
Contact List
Francisco Marco-Jiménez
contact affiliationInstituto de Ciencia y Tecnología Animal, Universidad Politècnica de València, Valencia, Spain
contact emailfmarco@dca.upv.es
lab head
Luz Valero
contact affiliationUniversitat de Valencia
contact emailmluz.valero@uv.es
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD001836
PRIDE project URI
Repository Record List
[ + ]