PXD001833

PXD001833 is an original dataset announced via ProteomeXchange.

Title	Immunohistochemical markers distinguishing cholangiocellular carcinoma from pancreatic ductal adenocarcinoma discovered by proteomic analysis of microdissected cells
Description	Cholangiocellular carcinoma (CCC) and pancreatic ductal adenocarcinoma (PDAC) are two highly aggressive cancer types which arise from epithelial cells of the pancreatobiliary system. Due to their histologic and morphologic similarity, differential diagnosis between CCC and metastasis of PDAC located in the liver frequently proves an unsolvable issue for pathologists. Biomarkers with high specificity and sensitivity for the differentiation of these tumour types would therefore be a valuable tool, but so far none are available. Here, we address this problem by comparing microdissected CCC and PDAC tumour cells in a label-free proteomics approach. The novel biomarker candidates were subsequently verified by immunohistochemical staining of primary and secondary tumour tissue.
HostingRepository	PRIDE
AnnounceDate	2015-12-10
AnnouncementXML	Submission_2015-12-10_02:44:43.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001833
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Juliet Padden
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-02-20 07:48:03	ID requested
⏵ 1	2015-12-10 02:44:45	announced

Padden J, Ahrens M, K, ä, lsch J, Bertram S, Megger DA, Bracht T, Eisenacher M, Kocabayoglu P, Meyer HE, Sipos B, Baba HA, Sitek B, Immunohistochemical Markers Distinguishing Cholangiocellular Carcinoma (CCC) from Pancreatic Ductal Adenocarcinoma (PDAC) Discovered by Proteomic Analysis of Microdissected Cells. Mol Cell Proteomics, 15(3):1072-82(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Cholangiocellular carcinoma, pancreatic ductal adenocarcinoma, biomarker, LC-MS/MS, laser capture microdissection

Barbara Sitek
contact affiliation	Medizinisches Proteom-Center - Ruhr-Universität Bochum
contact email	barbara.sitek@rub.de
lab head
Juliet Padden
contact affiliation	Medizinisches Proteom-Center - Ruhr-Universität Bochum
contact email	juliet.padden@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/12/PXD001833

PRIDE project URI

• PRIDE
  1. PXD001833
     1. Label: PRIDE project
     2. Name: Immunohistochemical markers distinguishing cholangiocellular carcinoma from pancreatic ductal adenocarcinoma discovered by proteomic analysis of microdissected cells