PXD001831

PXD001831 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteome of the cyanobacterium Synechocystis sp. PCC 6803 and its dynamics during nitrogen starvation
Description	Cyanobacteria have shaped the earth’s biosphere as the first oxygenic photoautotrophs and still play an important role in many ecosystems. The ability to adapt to changing environmental conditions is an essential characteristic in order to ensure survival. To this end, numerous studies have shown that bacteria use protein post-translational modifications such as Ser/Thr/Tyr phosphorylation in cell signalling, adaptation and regulation. Nevertheless, our knowledge of cyanobacterial phosphoproteomes and their dynamic response to environmental stimuli is relatively limited. In this study, we applied gel-free methods and high accuracy mass spectrometry towards the unbiased detection of Ser/Thr/Tyr phosphorylation events in the model cyanobacterium Synechocystis sp. PCC 6803. We could identify over 300 phosphorylation events in cultures grown on nitrate as exclusive nitrogen source. Chemical dimethylation labelling was applied to investigate proteome and phosphoproteome dynamics during nitrogen starvation. Our dataset describes the most comprehensive (phospho)proteome of Synechocystis to date, identifying 2,382 proteins and 183 phosphorylation events and quantifying 2,111 proteins and 148 phosphorylation events during nitrogen starvation. Global protein phosphorylation levels were increased in response to nitrogen depletion after 24 hours. Among the proteins with increased phosphorylation, the PII signalling protein showed the highest fold-change, serving as positive control. Other proteins with increased phosphorylation levels comprised functions in photosynthesis and in carbon and nitrogen metabolism. This study reveals dynamics of Synechocystis phosphoproteome in response to environmental stimuli and suggests an important role of protein Ser/Thr/Tyr phosphorylation in fundamental mechanisms of homeostatic control in cyanobacteria.
HostingRepository	PRIDE
AnnounceDate	2016-05-09
AnnouncementXML	Submission_2016-05-09_06:56:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philipp Spaet
SpeciesList	scientific name: Synechocystis sp. (strain PCC 6803 / Kazusa); NCBI TaxID: 1111708;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-02-20 05:13:47	ID requested
⏵ 1	2016-05-09 06:56:14	announced

Sp, ä, t P, Ma, č, ek B, Forchhammer K, Phosphoproteome of the cyanobacterium Synechocystis sp. PCC 6803 and its dynamics during nitrogen starvation. Front Microbiol, 6():248(2015) [pubmed]

curator keyword: Biological

submitter keyword: Cyanobacteria, Synechocystis sp. PCC 6803, Nitrogen starvation, Phosphoproteome, peptide dimethylation labeling, LTQ-Orbitrap

Boris Macek
contact affiliation	Prof. Dr. Boris Macek Proteome Center Tuebingen Interfaculty Institute for Cell Biology University of Tuebingen Auf der Morgenstelle 15 72076 Tuebingen Germany Phone: +49/(0)7071/29-70556 Fax: +49/(0)7071/29-5779 Web: http://www.pct.uni-tuebingen.de/
contact email	boris.macek@uni-tuebingen.de
lab head
Philipp Spaet
contact affiliation	University Tuebingen Department of Organismic Interactions
contact email	philipp.spaet@uni-tuebingen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001831
     1. Label: PRIDE project
     2. Name: Phosphoproteome of the cyanobacterium Synechocystis sp. PCC 6803 and its dynamics during nitrogen starvation