PXD001825

PXD001825 is an original dataset announced via ProteomeXchange.

Title	Aquired resistance mechanisms to EGFR-specific kinase inhibitors using chemical proteomics
Description	Here we used kinase affinity enrichment (Kinobeads) and intensity-based mass spectrometry to elucidate aquired resistance mechanisms to EGFR inhibition in cancer cells. First, kinase expression differences between untreated control cells and long term treated resistant cells were investigated using Kinobeads and LC-MS/MS. Overexpressed Kinases were then further investigated by cellular assays to evaluate targets and development of combination treatment strategies.
HostingRepository	PRIDE
AnnounceDate	2015-05-20
AnnouncementXML	Submission_2015-05-20_06:16:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Heiner Koch
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-02-19 02:26:01	ID requested
⏵ 1	2015-05-20 06:16:57	announced

Koch H, Busto ME, Kramer K, M, é, dard G, Kuster B, Chemical Proteomics Uncovers EPHA2 as a Mechanism of Acquired Resistance to Small Molecule EGFR Kinase Inhibition. J Proteome Res, 14(6):2617-25(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: LC-MS/MS, kinase inhibitor, drug resistance, cancer

Prof. Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich, Germany
contact email	kuster@tum.de
lab head
Heiner Koch
contact affiliation	Technische Universitaet Muenchen
contact email	heiner.koch@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/05/PXD001825

PRIDE project URI

• PRIDE
  1. PXD001825
     1. Label: PRIDE project
     2. Name: Aquired resistance mechanisms to EGFR-specific kinase inhibitors using chemical proteomics