PXD001824

PXD001824 is an original dataset announced via ProteomeXchange.

Title	Improved protease digestion conditions for membrane protein detection
Description	This work presents improved protease digestion conditions for membrane protein detection. Two improved conditions, 0.01% SDS or the combination of 10% MeOH and 0.01% RapiGest, were chosen and applied to proteins from Escherichia coli. The application of both improved conditions to a membrane protein fraction of Escherichia coli resulted in the identification of 309 (SDS) and 329 (MeOH/RapiGest) unique proteins of which 140/309 and 148/329 were membrane proteins.
HostingRepository	PRIDE
AnnounceDate	2015-04-28
AnnouncementXML	Submission_2015-04-28_02:30:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lie Min
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q TRAP

Revision	Datetime	Status	ChangeLog Entry
0	2015-02-18 01:00:08	ID requested
⏵ 1	2015-04-28 02:30:32	announced

Min L, Choe LH, Lee KH, Improved protease digestion conditions for membrane protein detection. Electrophoresis, 36(15):1690-8(2015) [pubmed]

curator keyword: Biological

submitter keyword: E. coli, membrane protein, proteomics

Kelvin H. Lee
contact affiliation	Delaware Biotechnology Institute, Department of Chemical and Biomolecular Engineering, University of Delaware
contact email	khl@udel.edu
lab head
Lie Min
contact affiliation	Department of Chemical and Biomolecular Engineering, Delaware Biotechnology Institute, University of Delaware
contact email	lmin@udel.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/04/PXD001824

PRIDE project URI

• PRIDE
  1. PXD001824
     1. Label: PRIDE project
     2. Name: Improved protease digestion conditions for membrane protein detection