PXD001824 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Improved protease digestion conditions for membrane protein detection |
Description | This work presents improved protease digestion conditions for membrane protein detection. Two improved conditions, 0.01% SDS or the combination of 10% MeOH and 0.01% RapiGest, were chosen and applied to proteins from Escherichia coli. The application of both improved conditions to a membrane protein fraction of Escherichia coli resulted in the identification of 309 (SDS) and 329 (MeOH/RapiGest) unique proteins of which 140/309 and 148/329 were membrane proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2015-04-28 |
AnnouncementXML | Submission_2015-04-28_02:30:30.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lie Min |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q TRAP |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-02-18 01:00:08 | ID requested | |
⏵ 1 | 2015-04-28 02:30:32 | announced | |
Publication List
Min L, Choe LH, Lee KH, Improved protease digestion conditions for membrane protein detection. Electrophoresis, 36(15):1690-8(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: E. coli, membrane protein, proteomics |
Contact List
Kelvin H. Lee |
contact affiliation | Delaware Biotechnology Institute, Department of Chemical and Biomolecular Engineering, University of Delaware |
contact email | khl@udel.edu |
lab head | |
Lie Min |
contact affiliation | Department of Chemical and Biomolecular Engineering, Delaware Biotechnology Institute, University of Delaware |
contact email | lmin@udel.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001824
- Label: PRIDE project
- Name: Improved protease digestion conditions for membrane protein detection