PXD001810

PXD001810 is an original dataset announced via ProteomeXchange.

Title	SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage
Description	Small Ubiquitin-like Modifiers play critical roles in the DNA Damage Response (DDR). To increase our understanding of SUMOylation in the mammalian DDR, we employed a quantitative proteomics approach to identify dynamically regulated SUMO-2 conjugates and modification sites upon treatment with the DNA damaging agent MMS. We have uncovered a dynamic set of 20 upregulated and 33 downregulated SUMO-2 conjugates, and 755 SUMO-2 sites, of which 362 were dynamic in response to MMS. In contrast to yeast, where a response is centered on homologous recombination, we identified dynamically SUMOylated interaction networks of chromatin modifiers, transcription factors, DNA repair factors and nuclear body components. SUMOylated chromatin modifiers include JARID1B/KDM5B, JARID1C/KDM5C, p300, CBP, PARP1, SetDB1 and MBD1. Whereas SUMOylated JARID1B was ubiquitylated by the SUMO-targeted ubiquitin ligase RNF4 and degraded by the proteasome in response to DNA damage, JARID1C was SUMOylated and recruited to the chromatin to demethylate histone H3K4.
HostingRepository	PRIDE
AnnounceDate	2015-03-16
AnnouncementXML	Submission_2015-03-17_06:18:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ivo Hendriks
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	sumoylated lysine
Instrument	Q Exactive; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-02-12 09:01:16	ID requested
1	2015-03-16 04:18:08	announced
⏵ 2	2015-03-17 06:18:24	announced	Updated publication reference for PubMed record(s): 25772364.

Hendriks IA, Treffers LW, Verlaan-de Vries M, Olsen JV, Vertegaal ACO, SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage. Cell Rep, 10(10):1778-1791(2015) [pubmed]

curator keyword: Biological

submitter keyword: SUMO-2, SUMO, MMS, DNA, damage, chromatin, repair, site

Alfred C.O. Vertegaal
contact affiliation	Department of Molecular Cell Biology, Leiden University Medical Center, The Netherlands
contact email	a.c.o.vertegaal@lumc.nl
lab head
Ivo Hendriks
contact affiliation	Leiden University Medical Center
contact email	ivo.a.hendriks@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/03/PXD001810

PRIDE project URI

• PRIDE
  1. PXD001810
     1. Label: PRIDE project
     2. Name: SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage