PXD001781

PXD001781 is an original dataset announced via ProteomeXchange.

Title	Charting the interactome of PDE3A in human cells using an IBMX based chemical proteomics approach
Description	We attempt to study the interactome of PDEs by synthesizing a broad spectrum PDE-capturing resin based on the non-selective PDE inhibitor 3-isobutyl-1-methylxanthine (IBMX). Chemical proteomics characterization of this resin in HeLa cell lysates led to the capture of several different PDEs. Combining the IBMX-resin with in-solution competition with other available, more selective PDE inhibitors, namely cilostamide and papaverine, allowed us to selectively probe the interactome of PDE3A in HeLa cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:19:38.524.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eleonora Corradini
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600; Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-02-04 08:43:40	ID requested
1	2016-05-25 09:25:48	announced
⏵ 2	2024-10-22 04:19:39	announced	2024-10-22: Updated project metadata.

Corradini E, Klaasse G, Leurs U, Heck AJ, Martin NI, Scholten A, Charting the interactome of PDE3A in human cells using an IBMX based chemical proteomics approach. Mol Biosyst, 11(10):2786-97(2015) [pubmed]

10.1039/c5mb00142k;

curator keyword: Technical, Biological

submitter keyword: IBMX, mass spectrometry, phosphodiesterase, papaverine, cilostamide,Chemical proteomics

Albert J.R. Heck
contact affiliation	Biomolecular Mass Spectrometry and Proteomics Group, Utrecht University, Utrecht, The Netherlands
contact email	a.j.r.heck@uu.nl
lab head
Eleonora Corradini
contact affiliation	Biomolecular and mass spectrometry group
contact email	e.corradini1@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001781

PRIDE project URI

• PRIDE
  1. PXD001781
     1. Label: PRIDE project
     2. Name: Charting the interactome of PDE3A in human cells using an IBMX based chemical proteomics approach