PXD001768

PXD001768 is an original dataset announced via ProteomeXchange.

Title	Differential analysis of dystrophic dog muscle after MuStem cell therapy
Description	Duchenne Muscular Dystrophy (DMD), the most common form of inherited neuromuscular disorder, is caused by mutations in the dystrophin gene leading to progressive muscle loss. Secondary changes in signaling pathways and energy metabolism are aggravating factors. Thus, for the evaluation of novel therapeutic approaches, it is essential to analyse the reversal of both primary and secondary abnormalities in treated muscle. MuStem cells constitute a new progenitor cell population proposed as a source of cells for a therapeutic strategy. When injected in Golden Retriever Muscular Dystrophy (GRMD) dogs, MuStem cells contribute to myofiber regeneration, satellite cell replenishment, and dystrophin expression. This cell therapy leads to muscle damage course limitation with an increased regeneration activity and an interstitial expansion restriction, and persisting stabilization of the dog's clinical status. In this study, we used isotope-coded protein labeling (ICPL) to compare the protein expression profiles of muscles coming from three different types of dogs: healthy, GRMD and GRMD after injection of MuStem cells. Based on the comparison of three biological replicates, these triplex ICPL experiments led to the identification of proteins differentially expressed after treatment. This study contributes to a better understanding of the mechanisms of action of MuStem cells.
HostingRepository	PRIDE
AnnounceDate	2019-05-24
AnnouncementXML	Submission_2019-05-24_06:10:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Melanie Lagarrigue
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-01-30 01:51:47	ID requested
1	2019-05-24 05:18:24	announced
⏵ 2	2019-05-24 06:10:38	announced	Updated publication reference for PubMed record(s): 27246553.

Lardenois A, Jagot S, Lagarrigue M, Gu, é, vel B, Ledevin M, Larcher T, Dubreil L, Pineau C, Rouger K, Gu, é, vel L, Quantitative proteome profiling of dystrophic dog skeletal muscle reveals a stabilized muscular architecture and protection against oxidative stress after systemic delivery of MuStem cells. Proteomics, 16(14):2028-42(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Duchenne Muscular Dystrophy, Golden Retriever Muscular dog, cell therapy, LC-MS/MS, ICPL

Charles Pineau
contact affiliation	Proteomics Core Facilities Biogenouest IRSET Inserm U1085 France
contact email	charles.pineau@inserm.fr
lab head
Melanie Lagarrigue
contact affiliation	Life Sciences
contact email	melanie.lagarrigue@univ-rennes1.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD001768

PRIDE project URI

• PRIDE
  1. PXD001768
     1. Label: PRIDE project
     2. Name: Differential analysis of dystrophic dog muscle after MuStem cell therapy