PXD001762

PXD001762 is an original dataset announced via ProteomeXchange.

Title	MoCBP3, an Antifungal Chitin-Binding Protein from Moringa oleifera Seeds, is a Member of the 2S Albumin Family
Description	MoCBP3 is a chitin-binding protein from M. oleifera seeds that inhibits the germination and mycelial growth of phytopathogenic fungi. This protein is highly thermostable and resistant to pH changes, and therefore may be useful in the development of new antifungal drugs. However, the relationship of MoCBP3 with the known families of carbohydrate-binding domains has not been established. In the present study, full-length cDNAs encoding 4 isoforms of MoCBP3 (MoCBP3-1, MoCBP3-2, MoCBP3-3 and MoCBP3-4) were cloned from developing seeds. The polypeptides encoded by the MoCBP3 cDNAs were predicted to contain 160 (MoCBP3-3) and 163 amino acid residues (MoCBP3-1, MoCBP3-2 and MoCBP3-4) with a signal peptide of 20-residues at the N-terminal region. A comparative analysis of the deduced amino acid sequences revealed that MoCBP3 is a typical member of the 2S albumin family, as shown by the presence of an eight-cysteine motif, which is a characteristic feature of the prolamin superfamily. Furthermore, mass spectrometry analysis demonstrated that MoCBP3 is a mixture of isoforms that correspond to different mRNA products. The identification of MoCBP3 as a genuine member of the 2S albumin family reinforces the hypothesis that these seed storage proteins are involved in plant defense. Moreover, the chitin-binding ability of MoCBP3 reveals a novel functionality for a typical 2S albumin.
HostingRepository	PRIDE
AnnounceDate	2016-05-25
AnnouncementXML	Submission_2016-05-25_11:54:01.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001762
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	FREDERICO MORENO
SpeciesList	scientific name: Moringa oleifera (Horseradish tree) (Moringa pterygosperma); NCBI TaxID: 3735;
ModificationList	Oxidation; Gln->pyro-Glu
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2015-01-29 01:40:14	ID requested
⏵ 1	2016-05-25 11:54:02	announced

Freire JE, Vasconcelos IM, Moreno FB, Batista AB, Lobo MD, Pereira ML, Lima JP, Almeida RV, Sousa AJ, Monteiro-Moreira AC, Oliveira JT, Grangeiro TB, Mo-CBP3, an antifungal chitin-binding protein from Moringa oleifera seeds, is a member of the 2S albumin family. PLoS One, 10(3):e0119871(2015) [pubmed]

curator keyword: Biological

submitter keyword: Moringa oleifera, Chitin-Binding

Thalles Barbosa Grangeiro
contact affiliation	Biology Departament. Federal University of Ceara. Brasil, Ceara.
contact email	tbgrangeiro@gmail.com
lab head
FREDERICO MORENO
contact affiliation	Dr. professor
contact email	pepeumoreno@yahoo.com.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001762
     1. Label: PRIDE project
     2. Name: MoCBP3, an Antifungal Chitin-Binding Protein from Moringa oleifera Seeds, is a Member of the 2S Albumin Family