PXD001759

PXD001759 is an original dataset announced via ProteomeXchange.

Title	Mouse Liver Proteins implicated in Metabolism and Type 2 Diabetes.
Description	While the performance of LC-SRM applied to samples from various bodily fluids, particularly plasma, and microorganisms has been extensively investigated,there is only little experience with its application to animal tissue Here, we show that a conventional one-dimensional LC-SRM workflow applied to mouse liver tissue suffers from a shortcoming in terms of sensitivity for lower abundance proteins. This problem could be solved through the extension of the standard workflow by an additional dimension of separation at the peptide level prior to online LC-SRM. For this purpose, we used off-gel electrophoresis (OGE) which is also shown to outperform strong cation exchange (SCX) in terms of resolution, gain of signal intensity, and predictability of separation. The extension of the SRM workflow by a high resolving peptide separation technique is an ideal combination as it allows the addition of stable isotope standards directly after trytic digestion and will increase the dynamic range of protein abundances amenable by SRM in animal tissue.
HostingRepository	PeptideAtlas
AnnounceDate	2015-01-29
AnnouncementXML	Submission_2015-01-29_13:04:55.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schäfer
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine
Instrument	4000 QTRAP

Revision	Datetime	Status	ChangeLog Entry
0	2015-01-28 15:27:47	ID requested
⏵ 1	2015-01-29 13:04:58	announced

no publication

curator keyword: selected reaction monitoring, SRM, targeted

Alexander Schäfer
contact affiliation	Helmholtz Center Munich
contact email	alexander.schaefer@helmholtz-muenchen.de
dataset submitter
Prof. Dr. Marius Ueffing
contact affiliation	Helmholtz Center Munich AND Institute for Ophthalmic Research at the University of Tübingen
contact email	marius.ueffing@uni-tuebingen.de
lab head

PeptideAtlas dataset URI

PASSEL experiment URI

PASSEL transition group browser URI