PXD001729

PXD001729 is an original dataset announced via ProteomeXchange.

Title	Quantitative Phosphoproteomic Identification of Novel Vasopressin-mediated Signaling Networks in Kidney Distal Convoluted Tubule Cells
Description	The kidney distal convoluted tubule (DCT) plays an important role in body sodium regulation and thus control of blood pressure. The main sodium reabsorption pathways in the DCT are the epithelial sodium channel (ENaC) and the thiazide-sensitive NaCl cotransporter (NCC), the functions of which can be modulated by the hormone vasopressin (VP) acting via uncharacterized signaling cascades. We performed large scale stable isotope labeling by amino acids in cell culture (SILAC) based quantitative phosphoproteomics of cultured mouse DCT cells (mpkDCT) to map global changes in protein phosphorylation events upon acute treatment with the VP type II receptor agonist 1-desamino-8-D-arginine vasopressin (dDAVP). The aim of this study is to identify unique VP signaling cascades in DCT cells that may be important for regulating ENaC or NCC activity. These pathways may be novel targets for modulation of blood pressure.
HostingRepository	PRIDE
AnnounceDate	2015-08-04
AnnouncementXML	Submission_2015-08-11_01:17:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qi Wu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; phosphorylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-01-26 01:21:35	ID requested
1	2015-08-04 13:54:37	announced
⏵ 2	2015-08-11 01:17:44	announced	Updated publication reference for PubMed record(s): 26239621.

Cheng L, Wu Q, Kortenoeven MLA, Pisitkun T, Fenton RA, A Systems Level Analysis of Vasopressin-mediated Signaling Networks in Kidney Distal Convoluted Tubule Cells. Sci Rep, 5():12829(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Quantitation, Phosphoproteomics, Vasopressin, Signaling Networks, Kidney Distal Convoluted Tubule Cells

Robert A. Fenton
contact affiliation	Center for Interactions of Proteins in Epithelial Transport, Department of Biomedicine, Aarhus University
contact email	robert.a.fenton@biomed.au.dk
lab head
Qi Wu
contact affiliation	Key Laboratory of Separation Science for Analytical Chemistry, CAS
contact email	qwu@dicp.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD001729

PRIDE project URI

• PRIDE
  1. PXD001729
     1. Label: PRIDE project
     2. Name: Quantitative Phosphoproteomic Identification of Novel Vasopressin-mediated Signaling Networks in Kidney Distal Convoluted Tubule Cells