PXD001728

PXD001728 is an original dataset announced via ProteomeXchange.

Title	Medicago truncatula drought stress recovery
Description	Molecular Elasticity and Adjustment of Drought Recovery Dynamics of 14N- and 15N-fertilized Legume Medicago truncatula. Climate change in conjunction with population growth necessitates a systems biology approach to characterize plant drought response and a more thorough understanding of the underlying molecular mechanisms. During drought stress and recovery, the metabolome and proteome regulate and are regulated through diverse mechanisms including synthesis and degradation. In order to study this complex regulation network, a front-end multilevel analysis is presented for the first time, investigating protein turnover, regulatory classes of proteins and metabolites as well as post translational ubiquitination of a target set of proteins during a severe stress and recovery scenario in the model legume Medicago truncatula. Evidence for enhanced translational proteome regulation was observed during drought recovery and functional clusters of differentially dynamic phases during the course of recovery were defined. The data give novel insights into molecular elasticity that enable recovery of drought stressed plants. Additionally, these results offer putative targets and metabolic pathways for future plant-bioengineering towards enhanced drought stress tolerance.
HostingRepository	PRIDE
AnnounceDate	2016-03-31
AnnouncementXML	Submission_2016-03-31_05:43:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Lyon
SpeciesList	scientific name: Medicago truncatula (Barrel medic) (Medicago tribuloides); NCBI TaxID: 3880;
ModificationList	ubiquitination signature dipeptidyl lysine; monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-01-26 01:21:12	ID requested
⏵ 1	2016-03-31 05:44:07	announced

Lyon D, Castillejo MA, Mehmeti-Tershani V, Staudinger C, Kleemaier C, Wienkoop S, Drought and Recovery: Independently Regulated Processes Highlighting the Importance of Protein Turnover Dynamics and Translational Regulation in Medicago truncatula. Mol Cell Proteomics, 15(6):1921-37(2016) [pubmed]

curator keyword: Biological

submitter keyword: Medicago truncatula, drought stress, drought recovery, time series, roots, shoots, non-nodulated, molecular systems biology, SELPEX

Stefanie Wienkoop
contact affiliation	Department for Ecogenomics and Systems Biology, University of Vienna, Austria
contact email	stefanie.wienkoop@univie.ac.at
lab head
David Lyon
contact affiliation	Ecogenomics and Systems Biology
contact email	david.lyon@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/03/PXD001728

PRIDE project URI

• PRIDE
  1. PXD001728
     1. Label: PRIDE project
     2. Name: Medicago truncatula drought stress recovery