PXD001723

PXD001723 is an original dataset announced via ProteomeXchange.

Title	Global proteomics analysis reveals a role for histone variant H3.3 in the response to starvation
Description	Periodic starvation of animals induces large shifts in metabolism, but may also influence many other cellular systems and can lead to adaption to prolonged starvation conditions. To date, there is limited understanding of how starvation affects gene expression, particularly at the protein level. Here, we have used mass spectrometry‐based quantitative proteomics to identify global changes in the C. elegans proteome due to acute starvation of young adult animals. Measuring changes in abundance of up to 7,000 proteins, we show that acute starvation rapidly alters the levels of hundreds of proteins, many involved in central metabolic pathways, highlighting key regulatory responses. Surprisingly, we also detect changes in the abundance of chromatin‐associated proteins including specific linker histones, histone variants and histone post‐translational modifications associated with the epigenetic control of gene expression. A null mutant for one of these proteins, the histone H3.3 variant HIS‐71, identified a subset of proteins whose abundance no longer varied in response to starvation. This mutant also displayed defects in starvation stress resistance and showed a reduced adult lifespan. To maximise community access to these data, they are presented in an online searchable database, the Encyclopedia of Proteome Dynamics (http://www.peptracker.com/epd/).
HostingRepository	PRIDE
AnnounceDate	2015-06-17
AnnouncementXML	Submission_2015-06-17_06:40:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vackar Afzal
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	N-ethylmaleimide derivatized cysteine; deamidated residue; monohydroxylated residue; acetylated residue; 2-pyrrolidone-5-carboxylic acid (Gln)
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-01-23 06:05:33	ID requested
⏵ 1	2015-06-17 06:40:05	announced

Larance M, Pourkarimi E, Wang B, Brenes Murillo A, Kent R, Lamond AI, Gartner A, Global Proteomics Analysis of the Response to Starvation in C. elegans. Mol Cell Proteomics, 14(7):1989-2001(2015) [pubmed]

curator keyword: Biological

submitter keyword: Histone, C. elegans, Starvation

Angus Lamond
contact affiliation	Lab Head
contact email	a.i.lamond@dundee.ac.uk
lab head
Vackar Afzal
contact affiliation	School of Life Sciences
contact email	v.z.afzal@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001723
     1. Label: PRIDE project
     2. Name: Global proteomics analysis reveals a role for histone variant H3.3 in the response to starvation