PXD001712

PXD001712 is an original dataset announced via ProteomeXchange.

Title	Gel-free mass spectrometry analysis of Drosophila melanogaster heads
Description	Here we describe a bottom-up approach to analyse an enriched membrane fraction from Drosophila melanogaster heads using multidimensional liquid chromatography (LC) coupled with tandem-mass spectrometry (MS/MS) that relies on a complete solubilisation and digestion of proteins. An enriched membrane fraction was prepared using equilibrium density centrifugation on a discontinual sucrose gradient, followed by solubilisation using FASP method, tryptic and consequential chymotryptic digestion of proteins. Peptides were separated by reversed-phase (RP) LC at high pH in the first dimension and acidic RP-LC coupled directly to Orbitrap Velos Pro mass spectrometer.
HostingRepository	PRIDE
AnnounceDate	2015-07-27
AnnouncementXML	Submission_2015-07-27_07:51:30.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001712
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jana Aradska
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-01-21 01:52:11	ID requested
⏵ 1	2015-07-27 07:51:32	announced

Aradska J, Bulat T, Sialana FJ, Birner-Gruenberger R, Erich B, Lubec G, Gel-free mass spectrometry analysis of Drosophila melanogaster heads. Proteomics, 15(19):3356-60(2015) [pubmed]

curator keyword: Biological

submitter keyword: proteomics, drosophila melanogaster, heads, membrane proteins, mass spectrometry

Gert Lubec
contact affiliation	Dept. of Pediatrics, Medical University of Wien, Austria
contact email	gert.lubec@meduniwien.ac.at
lab head
Jana Aradska
contact affiliation	MedUni Wien
contact email	jana.aradska@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/07/PXD001712

PRIDE project URI

• PRIDE
  1. PXD001712
     1. Label: PRIDE project
     2. Name: Gel-free mass spectrometry analysis of Drosophila melanogaster heads