PXD001692

PXD001692 is an original dataset announced via ProteomeXchange.

Title	Protein Structure Determination in Biological Matrices by Mass Spectrometry and Computational Biology
Description	We have implemented the use of a heterobifunctional, UV photoactivatable cross-linker, which greatly increases the number of identified cross-links compared with homobifunctional, NHS-ester based cross-linkers. We have cross-linked human serum albumin in the context of human blood serum. We present a novel methodology that combines the use of this high-resolution cross-linking with conformational space search to investigate the structure of proteins in their native environment.
HostingRepository	PRIDE
AnnounceDate	2015-09-22
AnnouncementXML	Submission_2015-09-22_04:30:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adam Belsom
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-01-19 01:18:12	ID requested
⏵ 1	2015-09-22 04:30:41	announced

Belsom A, Schneider M, Fischer L, Brock O, Rappsilber J, Serum Albumin Domain Structures in Human Blood Serum by Mass Spectrometry and Computational Biology. Mol Cell Proteomics, 15(3):1105-16(2016) [pubmed]

curator keyword: Biological, Technical

submitter keyword: Cross-linking and mass spectrometry, Human serum albumin

Juri Rappsilber
contact affiliation	Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, United Kingdom. Department of Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany.
contact email	juri.rappsilber@ed.ac.uk
lab head
Adam Belsom
contact affiliation	University of Edinburgh
contact email	adam.belsom@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/09/PXD001692

PRIDE project URI

• PRIDE
  1. PXD001692
     1. Label: PRIDE project
     2. Name: Protein Structure Determination in Biological Matrices by Mass Spectrometry and Computational Biology