PXD001689

PXD001689 is an original dataset announced via ProteomeXchange.

Title	A novel strategy of profiling protein methylation on lysine and arginine
Description	Protein methylation is involved in different processes including gene expression regulation, epigenetics, and nucleotide metabolism. Identification of methylated proteins is difficult as methyl groups are small, and do not introduce significant changes in protein hydrophobicity or charge state. The most effective analytical method to date is relative enrichment by pan-specific antibodies and methylation specific binding domains. Here, we present a novel and unbiased chemical strategy to enrich and identify sites of lysine and arginine methylations. This approach makes use of the property that methylation does not alter the charge state of lysine and arginine. This approach revealed over 793 methylation events including 211 arginine and 585 lysine methylation sites in HEK 293 Cells. This strategy proves to be convenient, effective and versatile, with the potential of analyzing other PTMs on both lysine and arginine.
HostingRepository	PRIDE
AnnounceDate	2016-08-16
AnnouncementXML	Submission_2016-08-16_01:45:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhibin Ning
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; acetylated residue; dimethylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-01-16 03:27:29	ID requested
⏵ 1	2016-08-16 01:45:15	announced

Ning Z, Star AT, Mierzwa A, Lanouette S, Mayne J, Couture JF, Figeys D, A charge-suppressing strategy for probing protein methylation. Chem Commun (Camb), 52(31):5474-7(2016) [pubmed]

curator keyword: Biological

submitter keyword: methylation

Daniel Figeys
contact affiliation	Professor, chair of BMI, faculty of Medicine, university of ottawa
contact email	dfigeys@uottawa.ca
lab head
Zhibin Ning
contact affiliation	research associate
contact email	ningzhibin@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD001689

PRIDE project URI

• PRIDE
  1. PXD001689
     1. Label: PRIDE project
     2. Name: A novel strategy of profiling protein methylation on lysine and arginine