PXD001683

PXD001683 is an original dataset announced via ProteomeXchange.

Title	Aeropyrum pernix K1 LC-MSMS
Description	The study investigated the ability of selected (hyper-)thermophilic prokaryotes to grow anaerobically by the reduction of perchlorate and chlorate. Physiological, genomic and proteome analyses suggest that the Crenarchaeon Aeropyrum pernix reduces (per)chlorate with a periplasmic enzyme related to nitrate reductases, while it lacks a functional chlorite-disproportionating enzyme (Cld). A. pernix seems to rely on the chemical reactivity of reduced sulfur compounds with the toxic intermediate chlorite to complete the pathway. The chemical oxidation of thiosulfate (in excessive amounts present in the medium) to sulfate and the concomitant release of chloride anions from the reduction of chlorite are the products of a biotic-abiotic (per)chlorate reduction pathway in A. pernix. The apparent absence of Cld in two other (per)chlorate-reducing microorganisms and their dependence on sulfide for (per)chlorate reduction is consistent with earlier-made observations on (per)chlorate-reducing Archaeoglobus fulgidus. All here discussed microorganisms use strategies for complete (per)chlorate reduction that differ from the physiology of classical (per)chlorate-reducing mesophiles.
HostingRepository	PRIDE
AnnounceDate	2017-06-02
AnnouncementXML	Submission_2017-06-01_23:34:59.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001683
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Martijn Pinkse
SpeciesList	scientific name: Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1); NCBI TaxID: 272557;
ModificationList	Ammonia-loss; Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-01-15 03:16:37	ID requested
⏵ 1	2017-06-01 23:35:00	announced

Liebensteiner MG, Pinkse MW, Nijsse B, Verhaert PD, Tsesmetzis N, Stams AJ, Lomans BP, Perchlorate and chlorate reduction by the Crenarchaeon Aeropyrum pernix and two thermophilic Firmicutes. Environ Microbiol Rep, 7(6):936-45(2015) [pubmed]

curator keyword: Biological

submitter keyword: Aeropyrum, Crenarchaeon, hyperthermophile, LC-MSMS

Martijn W. H. Pinkse
contact affiliation	Department of Biotechnology, Technical University Delft, Julianalaan 67. 2682 BC Delft, The Netherlands
contact email	m.w.h.pinkse@tudelft.nl
lab head
Martijn Pinkse
contact affiliation	Department of Biotechnology, Technical University Delft, Delft, The Netherlands
contact email	m.w.h.pinkse@tudelft.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD001683

PRIDE project URI

• PRIDE
  1. PXD001683
     1. Label: PRIDE project
     2. Name: Aeropyrum pernix K1 LC-MSMS