PXD001674

PXD001674 is an original dataset announced via ProteomeXchange.

Title	Immune suppressive neutrophils are a separate subset developing parallel to normal neutrophils
Description	Release of neutrophil subsets (hypersegmented, mature and banded) into the bloodstream was evoked by acute inflammation induced by iv. Injection of 2ng/kg lipopolysaccharide in healthy human volunteers pulse (6 hrs) labelled with deuteriated glucose. Three hours after injection, the three neutrophil subsets were sorted from blood. Incorporation of label into DNA was measured and proteome profiles were determined using high-end proteomics techniques (dimethyl labeling, SCX, nano-uplc, Q-Exactive).
HostingRepository	PRIDE
AnnounceDate	2017-05-29
AnnouncementXML	Submission_2017-05-29_05:46:08.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001674
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Patrick Wijten
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	dihydroxylated residue; 2x(13)C: 6x(2)H labeled dimethylated L-arginine; monohydroxylated residue; formylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-01-14 01:24:36	ID requested
⏵ 1	2017-05-29 05:46:09	announced

Tak T, Wijten P, Heeres M, Pickkers P, Scholten A, Heck AJR, Vrisekoop N, Leenen LP, Borghans JAM, Tesselaar K, Koenderman L, neutrophils identified as a separate subset by proteome profiling and in vivo pulse-chase labeling. Blood, 129(26):3476-3485(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, Neutrophils, LC-MS

Albert Heck
contact affiliation	Biomolecular Mass Spectrometry and Proteomics Group, Utrecht University, The Netherlands
contact email	A.J.R.Heck@uu.nl
lab head
Patrick Wijten
contact affiliation	Faculty of Science
contact email	P.wijten@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD001674

PRIDE project URI

• PRIDE
  1. PXD001674
     1. Label: PRIDE project
     2. Name: Immune suppressive neutrophils are a separate subset developing parallel to normal neutrophils