PXD001667
PXD001667 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | BioID-based identification of SCF-BTrCP1/2 E3 ligase substrates |
| Description | RAW files, mzML files, search results (gpm.xml), TPP analysis files (pep.XML and prot.XML, as well as tab delimited output of pep.XML as .xls) and analyzed results Table 1 for the identification of BTRCP1/2 substrates using BioID. The identification of ubiquitin E3 ligase substrates has been extremely challenging, due in part to low-affinity, transient interactions, the rapid degradation of targets, and the inability to identify proteins from poorly soluble cellular compartments. SCF-TrCP1 and SCF-TrCP2 are well studied ubiquitin E3 ligases that target substrates for proteasomal degradation, and play important roles in Wnt, Hippo and NFB signalling. Combining 26S proteasome inhibitor (MG132) treatment with proximity-dependent biotin labeling (BioID) and semi-quantitative mass spectrometry, here we identify SCF-TrCP1/2 interacting partners. Based on their enrichment in the presence of MG132, our data identify over 50 new putative SCF-TrCP1/2 substrates. We validate 12 of these new substrates, and reveal previously unsuspected roles for -TrCP in the maintenance of nuclear membrane integrity, processing (P)-body turnover, and translational control. Together, our data suggest that -TrCP is an important hub in the cellular stress response. The approach presented here should be broadly applicable for the identification of substrates for many ubiquitin E3 ligases. |
| HostingRepository | MassIVE |
| AnnounceDate | 2015-01-15 |
| AnnouncementXML | Submission_2015-01-15_03:15:59.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tharan Srikumar |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation; Dioxidation; Deamidated; Biotin |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2015-01-12 07:10:39 | ID requested | |
| ⏵ 1 | 2015-01-15 03:15:59 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: BioID, FLAG-IP, AP-MS, BTRCP, FBXW1, FBXW11 |
Contact List
| Brian Raught | |
|---|---|
| lab head | |
| Tharan Srikumar | |
| contact affiliation | UHN |
| contact email | tharan.srikumar@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://MSV000078991:a@massive.ucsd.edu |
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