PXD001662

PXD001662 is an original dataset announced via ProteomeXchange.

Title	Acetylome and succinylome of Corynebacterium glutamicum
Description	Profiles of two major acyl-modifications, lysine acetylation and succinylation, under L-glutamate-producting and non-producing conditions in Corynebacterium glutamicum, which is industrially utilized for amino acid fermentation, was analyzed. During glutamate overproduction induced by Tween 40, global lysine acetylation was decreased, while lysine succinylation was increased. A label-free semi-quantitative proteomic analysis identified 591 acetylated proteins with 1,509 unique acetylation sites and 297 succinylated proteins with 790 unique succinylation sites. Lysine acetylation and succinylation targeted most enzymes in the central carbon metabolic pathways that are directly related to glutamate production, including the 2-oxoglutarate dehydrogenase complex (ODHC), a key enzyme for glutamate overproduction.
HostingRepository	PRIDE
AnnounceDate	2015-12-21
AnnouncementXML	Submission_2015-12-21_05:54:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Saori Kosono
SpeciesList	scientific name: Corynebacterium glutamicum (strain ATCC 13032 / K051); NCBI TaxID: 1204414;
ModificationList	acetylated residue; iodoacetamide derivatized residue; N6-succinyl-L-lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-01-09 01:21:25	ID requested
⏵ 1	2015-12-21 05:54:22	announced

Mizuno Y, Nagano-Shoji M, Kubo S, Kawamura Y, Yoshida A, Kawasaki H, Nishiyama M, Yoshida M, Kosono S, Altered acetylation and succinylation profiles in Corynebacterium glutamicum in response to conditions inducing glutamate overproduction. Microbiologyopen, 5(1):152-73(2016) [pubmed]

curator keyword: Biological

submitter keyword: Corynebacterium glutamicum, glutamate production, LC-MS/MS, acetylome, succinylome

Saori Kosono
contact affiliation	Biotechnology Research Center, the University of Tokyo, Japan
contact email	uskos@mail.ecc.u-tokyo.ac.jp
lab head
Saori Kosono
contact affiliation	UTokyo
contact email	uskos@mail.ecc.u-tokyo.ac.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001662
     1. Label: PRIDE project
     2. Name: Acetylome and succinylome of Corynebacterium glutamicum