PXD001652

PXD001652 is an original dataset announced via ProteomeXchange.

Title	Mitochondrial Protein Hyperacetylation in the Failing Heart
Description	Mitochondrial proteomics was used to identify energy metabolic derangements that occur during the early stages of heart failure in well-defined mouse models. Levels of β-hydroxybutyrate dehydrogenase 1 (BDH1), a key enzyme in ketone oxidation, was upregulated. 13C-substrate flux studies and metabolomic profiling confirmed that the hypertrophied and early stage failing heart shifts to ketone bodies as a fuel source in the context of reduced oxidation of fatty acids, the chief substrate for the normal heart. This fuel shift is associated with an expansion of the acetyl pool and reduced levels of NAD+ levels resulting in increased mitochondrial protein acetylation. Myocardium of humans with heart failure also exhibited mitochondrial protein hyperacetylation. We propose that a shift to ketones as a fuel source leads to maladaptive bioenergetic consequences during the development of heart failure.
HostingRepository	PRIDE
AnnounceDate	2019-02-12
AnnouncementXML	Submission_2019-02-12_07:49:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ola Martin
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-01-06 01:17:45	ID requested
1	2016-02-26 02:13:50	announced
⏵ 2	2019-02-12 07:49:16	announced	Updated publication reference for PubMed record(s): 26998524.

Horton JL, Martin OJ, Lai L, Riley NM, Richards AL, Vega RB, Leone TC, Pagliarini DJ, Muoio DM, Bedi KC, Margulies KB, Coon JJ, Kelly DP, Mitochondrial protein hyperacetylation in the failing heart. JCI Insight, 2(1):(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Heart failure, acetylation, mitochondria

Daniel P. Kelly; Joshua J. Coon
contact affiliation	Kelly: Diabetes and Obesity Research Center, Sanford-Burnham Medical Research Institute, Orlando, Florida, USA. Coon: Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA Genome Center, University of Wisconsin-Madison, Madison, WI 53706, USA
contact email	dkelly@sanfordburnham.org
lab head
Ola Martin
contact affiliation	Sanford-Burnham Medical Research Institute
contact email	omartin@burnham.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD001652

PRIDE project URI

• PRIDE
  1. PXD001652
     1. Label: PRIDE project
     2. Name: Mitochondrial Protein Hyperacetylation in the Failing Heart