PXD001641

PXD001641 is an original dataset announced via ProteomeXchange.

Title	Single muscle fiber proteomics reveals unexpected mitochondrial specialization
Description	Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here we developed a high sensitivity workflow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and unbiased proteomics methods yielded the same subtype assignment. We discovered novel subtype specific features, most prominently mitochondrial specialization of fiber types in substrate utilization. The fiber type resolved proteomes can be applied to a variety of physiological and pathological conditions and illustrate the utility of single cell type analysis for dissecting proteomic heterogeneity.
HostingRepository	PRIDE
AnnounceDate	2015-02-05
AnnouncementXML	Submission_2015-02-05_04:11:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-01-02 02:48:40	ID requested
⏵ 1	2015-02-05 04:11:09	announced

Murgia M, Nagaraj N, Deshmukh AS, Zeiler M, Cancellara P, Moretti I, Reggiani C, Schiaffino S, Mann M, Single muscle fiber proteomics reveals unexpected mitochondrial specialization. EMBO Rep, 16(3):387-95(2015) [pubmed]

curator keyword: Biological, Technical

submitter keyword: exercise, metabolism, mitochondria, muscle fibers, single cell

Matthias Mann
contact affiliation	Max Planck Institute of Biochemistry Department of Proteomics and Signal Transduction Germany
contact email	mmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/02/PXD001641

PRIDE project URI

• PRIDE
  1. PXD001641
     1. Label: PRIDE project
     2. Name: Single muscle fiber proteomics reveals unexpected mitochondrial specialization