PXD001630

PXD001630 is an original dataset announced via ProteomeXchange.

Title	Age-related cleavages of crystallins in human lens cortical fibre cells generate a plethora of endogenous peptides and high molecular weight complexes
Description	Age-related breakdown of lenticular crystallins is associated with lenticular disorders such as cataract. Despite playing a critical role in maintaining lens homeostasis, the mechanism(s) and consequences of this phenomenon are not well understood. Utilising a proteomic-based approach, this study characterised 238 endogenous peptides derived from age-related crystallin breakdowns present in the cortical tissues of young, middle-age and old human lenses. Quantitative mass spectrometry analysis showed that the concentration of a prominent crystallin breakdown product in the lens increased significantly with age, which, coupled with the age-related increase in variety of the LMW crystallin peptide in the lens cortex, suggests that a major crystallin breakdown event taking place in the human lens cortex shortly after middle-age. In-depth analysis on the crystallin peptide terminal amino acids indicate the presence of trypsin-like proteolysis in the lens cortical cells, providing useful information on the mechanism(s) that contribute to crystallin breakdown in the aging human lens. Taken together, this work enhances our understanding on the age-related crystallin breakdown process in the cortical tissues of the human lens.
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_11:26:22.988.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001630
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Xiaomin Song
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation
Instrument	QSTAR

Revision	Datetime	Status	ChangeLog Entry
0	2014-12-19 03:18:22	ID requested
⏵ 1	2021-03-04 11:26:23	announced

Su SP, Song X, Xavier D, Aquilina JA, Age-related cleavages of crystallins in human lens cortical fiber cells generate a plethora of endogenous peptides and high molecular weight complexes. Proteins, 83(10):1878-86(2015) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Lens aging

crystallin

cataract

mass spectrometry

endogenous peptides

2-D gel electrophoresis

nanoLC-ESI-MS/MS

trypsin-like cleavage.

Xiaomin Song
contact affiliation	Australian Proteome Analysis Facility
contact email	xsong@proteome.org.au
lab head
Xiaomin Song
contact affiliation	APAF
contact email	xsong@proteome.org.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001630
     1. Label: PRIDE project
     2. Name: Age-related cleavages of crystallins in human lens cortical fibre cells generate a plethora of endogenous peptides and high molecular weight complexes