PXD001626

PXD001626 is an original dataset announced via ProteomeXchange.

Title	Widespread somatic L1 retrotransposition occurs early during gastrointestinal cancer evolution
Description	Somatic L1 retrotransposition events have been shown to occur in epithelial cancers1-8. Here, we attempted to determine how early somatic L1 insertions occurred during the development of gastrointestinal (GI) cancers. Using L1-targeted resequencing (L1-seq), we studied different stages of four colorectal cancers arising from colonic polyps, seven pancreatic carcinomas, as well as seven gastric cancers. Surprisingly, we found somatic L1 insertions not only in all cancer types and metastases, but also in colonic adenomas, well-known cancer precursors. Some insertions were also present in low quantities in normal GI tissues, occasionally caught in the act of being clonally fixed in the adjacent tumors. Insertions in adenomas and cancers numbered in the hundreds and many were present in multiple tumor sections implying clonal distribution. Our results demonstrate that extensive somatic insertional mutagenesis occurs very early during the development of GI tumors, probably before dysplastic growth. We assessed the impact of somatic L1 insertions on the expression of the corresponding protein-coding genes by comparing protein abundance in the polyp with the highest number of somatic L1 insertions with that of its paired normal colon using mass spectrometry analysis. Of the 10 validated somatic insertions that were in protein coding regions in the polyp, two proteins – KIAA1217 and WARS2 – were downregulated in the adenoma >90% and >70%, respectively.
HostingRepository	PRIDE
AnnounceDate	2019-02-12
AnnouncementXML	Submission_2019-02-12_07:48:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Szilvia Solyom
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2014-12-19 02:28:39	ID requested
1	2015-08-11 02:40:42	announced
⏵ 2	2019-02-12 07:48:09	announced	Updated publication reference for PubMed record(s): 6260970.

Espejo RT, L, ó, pez S, Arias C, Structural polypeptides of simian rotavirus SA11 and the effect of trypsin. J Virol, 37(1):156-60(1981) [pubmed]

curator keyword: Biomedical

submitter keyword: Retrotransposon, L1, adenoma, carcinoma, colorectal cancer

Haig H. Kazazian, Jr.
contact affiliation	McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 USA
contact email	hkazazi1@jhmi.edu
lab head
Szilvia Solyom
contact affiliation	Johns Hopkins University School of Medicine
contact email	ssolyom1@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001626
     1. Label: PRIDE project
     2. Name: Widespread somatic L1 retrotransposition occurs early during gastrointestinal cancer evolution