PXD001617

PXD001617 is an original dataset announced via ProteomeXchange.

Title	Global gene expression profiling through the complete life cycle of Trypanosoma vivax
Description	The parasitic flagellate Trypanosoma vivax is a cause of animal trypanosomiasis across Africa and South America. The parasite has a digenetic life cycle, passing between mammalian hosts and insect vectors, and a series of developmental forms adapted to each life cycle stage. Transcriptomic and proteomic studies of the related parasites T. brucei and T. congolense have shown how gene expression is regulated during their development. New methods for in vitro culture of the T. vivax insect stages have allowed us to describe global gene expression throughout the complete T. vivax life cycle for the first time. We combined transcriptomic and proteomic analysis of each life stage using RNA-seq and mass spectrometry respectively, to identify genes with patterns of preferential transcription or expression. While T. vivax is similar to related species in several ways, (e.g. developmental regulation of energy metabolism, restricted expression of a dominant variant antigen, and expression of ‘Fam50’ proteins in the insect mouthparts), we identify significant differences in gene expression affecting metabolism in the fly and a suite of T. vivax-specific genes with predicted cell-surface expression that are preferentially expressed in the mammal (‘Fam29, 30’) or the vector (‘Fam34, 35, 43’). Thus, T. vivax differs significantly from other African trypanosomes in the developmentally-regulated proteins it expresses on its cell surface and thus, in the structure of the host-parasite interface. These unique features may yet explain the species differences in life cycle and could, in the shape of bloodstream-stage proteins that do not undergo antigenic variation, provide targets for therapy.
HostingRepository	PRIDE
AnnounceDate	2019-02-12
AnnouncementXML	Submission_2019-02-12_07:47:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dong Xia
SpeciesList	scientific name: Trypanosoma vivax (strain Y486); NCBI TaxID: 1055687;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-12-16 02:50:32	ID requested
1	2015-07-20 07:24:50	announced
⏵ 2	2019-02-12 07:47:05	announced	Updated publication reference for PubMed record(s): 26266535.

Jackson AP, Goyard S, Xia D, Foth BJ, Sanders M, Wastling JM, Minoprio P, Berriman M, Global Gene Expression Profiling through the Complete Life Cycle of Trypanosoma vivax. PLoS Negl Trop Dis, 9(8):e0003975(2015) [pubmed]

curator keyword: Biological

submitter keyword: Complete life cycle, Trypanosoma vivax

Jonathan Wastling
contact affiliation	Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool
contact email	wasj@liv.ac.uk
lab head
Dong Xia
contact affiliation	Infection Biology
contact email	d.xia@liv.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001617
     1. Label: PRIDE project
     2. Name: Global gene expression profiling through the complete life cycle of Trypanosoma vivax