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PXD001611

PXD001611 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDefining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium
DescriptionIdentifying all essential genomic components is critical for the assembly of minimal artificial life. In the genome-reduced bacterium Mycoplasma pneumoniae, we found, that small ORFs (smORFs; <100 residues), accounting for 10% of all ORFs, are the most frequently essential genomic components (53%). Essentiality of smORFs may be explained by their function as members of protein and/or DNA/RNA complexes. In larger proteins, essentiality applied to individual domains and not entire proteins, a notion we could confirm by expression of truncated domains. The fraction of essential non-coding RNAs non-overlapping with essential genes is 5% higher than of non-transcribed regions (0.9%), pointing to the important functions of the former. The data highlights the minimal genome (33%, 269410 bp of M. pneumoniae genome), with an unexpected hidden layer of smORFs with essential functions.
HostingRepositoryPRIDE
AnnounceDate2015-03-03
AnnouncementXMLSubmission_2015-03-03_10:50:42.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJavier Delgado
SpeciesList scientific name: Mycoplasma pneumoniae M29; NCBI TaxID: 1441379;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-12-15 07:37:53ID requested
12015-03-03 10:50:43announced
Publication List
Lluch-Senar M, Delgado J, Chen WH, Llor, é, ns-Rico V, O'Reilly FJ, Wodke JA, Unal EB, Yus E, Mart, í, nez S, Nichols RJ, Ferrar T, Vivancos A, Schmeisky A, St, ü, lke J, van Noort V, Gavin AC, Bork P, Serrano L, Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium. Mol Syst Biol, 11(1):780(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: minimal genome, small proteins, non-coding RNAs
Contact List
Luis Serrano
contact affiliationEMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG), Dr. Aiguader 88, 08003 Barcelona, Spain
contact emailluis.serrano@crg.eu
lab head
Javier Delgado
contact affiliationCRG
contact emailjavier.delgado@crg.eu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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