PXD001606

PXD001606 is an original dataset announced via ProteomeXchange.

Title	California sea lion plasma chronic DAT biomarkers via 2D-GE
Description	Domoic acid toxicosis (DAT) in California sea lions (Zalophus californianus) is caused by exposure to the marine biotoxin domoic acid and has been linked to massive stranding events and mortality. Diagnosis is based on clinical signs in addition to the presence of domoic acid in body fluids. Chronic DAT further is characterized by reoccurring seizures progressing to status epilepticus. Diagnosis of chronic DAT is often slow and problematic, and minimally invasive tests for DAT have been the focus of numerous recent biomarker studies. The goal of this study was to retrospectively profile plasma proteins in a population of sea lions with chronic DAT and those without DAT using two dimensional gel electrophoresis in an effort to discover whether individual, multiple, or combinations of protein and clinical data could be utilized to identify sea lions with DAT. 49 spots were excised, digested and analyzed by MS/MS. These are the data.
HostingRepository	PRIDE
AnnounceDate	2015-05-07
AnnouncementXML	Submission_2015-05-07_04:27:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Neely
SpeciesList	scientific name: Zalophus californianus (California sealion); NCBI TaxID: 9704;
ModificationList	deamidated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2014-12-15 01:47:32	ID requested
⏵ 1	2015-05-07 04:27:34	announced

Neely BA, Ferrante JA, Chaves JM, Soper JL, Almeida JS, Arthur JM, Gulland FM, Janech MG, Proteomic Analysis of Plasma from California Sea Lions (Zalophus californianus) Reveals Apolipoprotein E as a Candidate Biomarker of Chronic Domoic Acid Toxicosis. PLoS One, 10(4):e0123295(2014) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: sea lion, 2D-GE, plasma, depletion, ApoE

Michael Gregory Janech
contact affiliation	Department of Medicine, Division of Nephrology, Medical University of South Carolina, Charleston, SC, USA
contact email	janechmg@musc.edu
lab head
Benjamin Neely
contact affiliation	Medical University of South Carolina
contact email	benaneely@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/04/PXD001606

PRIDE project URI

• PRIDE
  1. PXD001606
     1. Label: PRIDE project
     2. Name: California sea lion plasma chronic DAT biomarkers via 2D-GE