PXD001603

PXD001603 is an original dataset announced via ProteomeXchange.

Title	Signaling pathways altered at the mitotic spindle in clathrin depleted cells
Description	We present a snapshot of the regulation of mitotic-spindle proteins and their phosphorylation status in the absence of clathrin during metaphase. A reference list of proteins and phosphosites identified from a mitotic spindle preparation was generated using a label-free and a parallel chemical dimethyl isotopic peptide labelling approach. We further designed experiments to analyse proteins and phosphosites at the mitotic spindle whose abundance was affected by the knock-down of clathrin. We used dimethylation labelling of peptides in combination with fractionation strategies to identify and quantify phosphopeptides enriched by TiO2 beads and the flow-through non-phoshopeptides by ESI-LC-MS/MS for a broad coverage of the mitotic spindle proteome in the absence of clathrin.
HostingRepository	PRIDE
AnnounceDate	2016-05-17
AnnouncementXML	Submission_2016-05-17_10:50:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sushma Rao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deamidated residue; monohydroxylated residue; acetylated residue; phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-12-12 07:39:25	ID requested
⏵ 1	2016-05-17 10:50:21	announced

Rao SR, Flores-Rodriguez N, Page SL, Wong C, Robinson PJ, Chircop M, The Clathrin-dependent Spindle Proteome. Mol Cell Proteomics, 15(8):2537-53(2016) [pubmed]

curator keyword: Biological

submitter keyword: HeLa, Dimethylation, CHC knock-down, TiO2, high pH reverse phase, SCX, LC-MS/MS

A/Prof. Megan Chircop
contact affiliation	Cell Cycle Unit, Children's Medical Research Institute, 214 Hawkesbury Road, Westmead, NSW 2145, Australia
contact email	mchircop@cmri.org.au
lab head
Sushma Rao
contact affiliation	Children's Medical Research Institute
contact email	srao@cmri.org.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001603

PRIDE project URI

• PRIDE
  1. PXD001603
     1. Label: PRIDE project
     2. Name: Signaling pathways altered at the mitotic spindle in clathrin depleted cells