PXD001601

PXD001601 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis to study the involvement of the Tor pathway in the human-pathogenic fungus Aspergillus fumigatus
Description	The Tor kinase is one of the major regulatory nodes in a eukaryotic cell and until now, little is known about this kinase in filamentous fungi. Here, we analysed the Tor kinase in Aspergillus fumigatus, which is the most important airborne fungal pathogen of humans and the main causative agent of invasive aspergillosis. Because deletion of the single tor gene of A. fumigatus was apparently lethal, we generated a conditional lethal tor mutant. This was achieved by fusing the tor gene with the inducible xylose promoter and replacing the endogenous tor gene by the inducible xylp-tor gene cassette. The generated transgenic strain opened up the possibility to activate or silence the tor gene under controlled conditions. Since we did not observe any effect of rapamycin on Tor of A. fumigatus, the generation of the inducible Tor gene was even more valuable for an in-depth analysis of the Tor kinase. By LC-MS/MS analyses of mycelial proteins we identified the regulatory network of Tor. Tor controls a variety of genes and proteins involved in nutrient sensing, stress response, cell cycle progression, protein biosynthesis and degradation. Tor is a major kinase responsible for protein import into the mitochondria, and consequently responsible for the correct function of most processes occurring into these organelles, e.g. respiration and ornithine metabolism. Regulation of iron acquisition by Tor was found to be independent of the HapX transcription factor.
HostingRepository	PRIDE
AnnounceDate	2022-02-28
AnnouncementXML	Submission_2022-02-28_02:25:44.928.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Krueger
SpeciesList	scientific name: Aspergillus fumigatus CEA10; NCBI TaxID: 505235;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-12-12 05:12:08	ID requested
⏵ 1	2022-02-28 02:25:45	announced

Baldin C, Valiante V, Kr, ü, ger T, Schafferer L, Haas H, Kniemeyer O, Brakhage AA, Comparative proteomics of a tor inducible Aspergillus fumigatus mutant reveals involvement of the Tor kinase in iron regulation. Proteomics, 15(13):2230-43(2015) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: LC-MS/MS

TMT, Tor kinase, fungi, proteomics

Dr. Olaf Kniemeyer
contact affiliation	Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute Jena Department of Molecular and Applied Microbiology Subgroup Stress- and Immunoproteomics
contact email	olaf.kniemeyer@hki-jena.de
lab head
Thomas Krueger
contact affiliation	Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute Jena
contact email	thomas.krueger@hki-jena.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001601
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis to study the involvement of the Tor pathway in the human-pathogenic fungus Aspergillus fumigatus