PXD001597

PXD001597 is an original dataset announced via ProteomeXchange.

Title	In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation
Description	Microglia, astrocytes, and neurons, which have important functions in the central nervous system, communicate mutually to generate a signal through secreted proteins or small molecules, but many of which have not been identified. Because establishing a reference for the secreted proteins from CNS cells could be invaluable in examining cell-to-cell communication in the brain, we analyzed the secretome of 3 murine CNS cell lines without pre-fractionation by high-resolution mass spectrometry. In this study, 2795 proteins were identified from conditioned media of the 3 cell lines, and 2125 proteins were annotated as secreted proteins by bioinformatics analysis. Further, approximately 500 secreted proteins were quantifiable as differentially expressed proteins by label-free quantitation. As a result, our secretome references are useful datasets for the future study of neuronal diseases.
HostingRepository	PRIDE
AnnounceDate	2015-08-04
AnnouncementXML	Submission_2015-08-04_09:53:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jongmin Woo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-12-12 01:31:47	ID requested
⏵ 1	2015-08-04 09:53:38	announced

Woo J, Han D, Park J, Kim SJ, Kim Y, In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation. Proteomics, 15(21):3617-22(2015) [pubmed]

curator keyword: Biological

submitter keyword: CNS cell line, proteome profile, secretome, label-free quantitation, neuron, microglia, astrocyte

Youngsoo Kim
contact affiliation	institute of medical and biological engineering, Seoul National University College of Medicine
contact email	biolab@snu.ac.kr
lab head
Jongmin Woo
contact affiliation	Seoul National University
contact email	jongminw@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD001597

PRIDE project URI

• PRIDE
  1. PXD001597
     1. Label: PRIDE project
     2. Name: In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation