PXD001584

PXD001584 is an original dataset announced via ProteomeXchange.

Title	Importance of arginine trasporter ArgP in Francisella by comparative proteomics.
Description	Metabolic adaptation has recently been recognized as a key component of the intracellular life cycle of pathogenic microorganisms. Upon entry into mammalian host cells, Francisella tularensis, a pathogenic bacterium responsible for the zoonotic disease tularemia, rapidly escapes from the phagosome to multiply actively in the cytosolic compartment. Francisella is auxotroph for several amino acids, including arginine, and thus relies exclusively on their import from the host to survive in infected cells. We identified an arginine transporter (hereafter designated ArgP) whose inactivation considerably delayed bacterial phagosomal escape and intracellular multiplication. Remarkably, this defect could be totally suppressed in macrophages and dendritic cells from IRAP-KO mice. Wild-type cytosolic multiplication of the mutant bacteria was restored in these cells, indicating that the ArgP transporter is mainly required for arginine uptake in the phagosomal compartment. Proteomic analyses showed that arginine deprivation in the ∆argP mutant rapidly affected the expression of a numerous proteins, and in particular reduced expression of most of the ribosomal proteins. We propose that regulation of ribosomal protein biogenesis may contribute to the sensing of the intracellular stage of the pathogen and to phagosomal membrane disruption.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_00:15:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara guerrera
SpeciesList	scientific name: Francisella tularensis subsp. novicida (strain U112); NCBI TaxID: 401614;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-12-08 06:09:03	ID requested
1	2015-01-28 02:23:57	announced
2	2017-06-21 00:30:53	announced	Updated project metadata.
⏵ 3	2017-10-24 00:15:03	announced	Updated project metadata.

Ramond E, Gesbert G, Guerrera IC, Chhuon C, Dupuis M, Rigard M, Henry T, Barel M, Charbit A, Importance of host cell arginine uptake in Francisella phagosomal escape and ribosomal protein amounts. Mol Cell Proteomics, 14(4):870-81(2015) [pubmed]

curator keyword: Biological

submitter keyword: F. Tularensis, Labelfree, Q Exactive Plus, Arginine transporter

Ida Chiara GUERRERA
contact affiliation	Necker proteomic platform, , PPN-3P5, Structure Federative de Recherche Necker, INSERM US24,
contact email	chiara.guerrera@inserm.fr
lab head
Chiara guerrera
contact affiliation	Faculte de Medecine
contact email	chiara.guerrera@inserm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001584
     1. Label: PRIDE project
     2. Name: Importance of arginine trasporter ArgP in Francisella by comparative proteomics.