PXD001581

PXD001581 is an original dataset announced via ProteomeXchange.

Title	The proteome of human retrobulbar optic nerve
Description	The optic nerve is a white matter tract that conveys visual information to the brain. A detailed investigation of the proteome of the normal human retrobulbar optic nerve may help facilitate studies of the biology and pathophysiology of the optic nerve. We conducted an in-depth proteomic analysis of optic nerve from five adults. Proteins were fractionated using SDS-PAGE. After in-gel digestion, peptides were analyzed using LC-MS/MS on an Orbitrap Elite mass spectrometer. We identified 2,711 non-redundant proteins in the human retrobulbar optic nerve, including the astrocytic marker glial fibrillary acidic protein, several proteins expressed by oligodendrocytes (laminin, proteolipid protein, and fibronectin), myelin proteins (myelin basic protein, myelin-associated glycoprotein), paranodal structural proteins (neurofascin, contactin, α, β, and γ adducins, septin 2, endophilin, ankyrin β, spectrin), proteins involved in neuronal protection and regeneration (α crytallins A and B, dedicator of cytokinesis proteins, ciliary neurotrophic factor), proteins associated with open-angle glaucoma (thioredoxin, heat shock protein-70), and proteins associated with optic neuritis (aquaporin-4). Twenty-one unambiguous protein isoforms were identified in the optic nerve.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:21:52.929.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pingbo Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2014-12-08 02:13:26	ID requested
1	2016-08-24 04:26:51	announced
⏵ 2	2024-10-22 04:21:55	announced	2024-10-22: Updated project metadata.

Zhang P, Karani R, Turner RL, Dufresne C, Ferri S, Van Eyk JE, Semba RD, The proteome of normal human retrobulbar optic nerve and sclera. Proteomics, 16(19):2592-2596(2016) [pubmed]

10.1002/pmic.201600229;

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

curator keyword: Biological, Biomedical

submitter keyword: Eye / Human / Optic Nerve / Proteome

Richard D. Semba
contact affiliation	Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD
contact email	rdsemba@jhmi.edu
lab head
Pingbo Zhang
contact affiliation	Johns Hopkins University
contact email	pzhang7@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001581
     1. Label: PRIDE project
     2. Name: The proteome of human retrobulbar optic nerve